Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI (3474) 1 site
TGGCCAACCGGT
* Blocked by Dcm methylation.
BseRI (3353) 1 site
GAGGAG(N)8NNCTCCTC(N)8
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
SalI (3108) 1 site
GTCGACCAGCTG
NaeI (2909) 1 site
GCCGGCCGGCCG
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV (2907) 1 site
GCCGGCCGGCCG
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII (2806) 1 site
CACNNNGTGGTGNNNCAC
Sticky ends from different DraIII sites may not be compatible.
XmnI (2263) 1 site
GAANNNNTTCCTTNNNNAAG
BamHI (79) 1 site
GGATCCCCTAGG
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BspDI (87) 1 site
ATCGATTAGCTA
ClaI (87) 1 site
ATCGATTAGCTA
BstBI (92) 1 site
TTCGAAAAGCTT
EcoRI (94) 1 site
GAATTCCTTAAG
StuI (103) 1 site
AGGCCTTCCGGA
AvaI (107) 1 site
CYCGRGGRGCYC
Sticky ends from different AvaI sites may not be compatible.
BsoBI (107) 1 site
CYCGRGGRGCYC
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I (107) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI (107) 1 site
CTCGAGGAGCTC
BmeT110I (108) 1 site
CYCGRGGRGCYC
XbaI (115) 1 site
TCTAGAAGATCT
SnaBI (140) 1 site
TACGTAATGCAT
HpaI (278) 1 site
GTTAACCAATTG
MfeI (287) 1 site
CAATTGGTTAAC
EagI (346) 1 site
CGGCCGGCCGGC
NotI (346) 1 site
GCGGCCGCCGCCGGCG
BtgI (349) 1 site
CCRYGGGGYRCC
Sticky ends from different BtgI sites may not be compatible.
SacII (352) 1 site
CCGCGGGGCGCC
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI (364) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
NsiI (364) 1 site
ATGCATTACGTA
PspOMI (367) 1 site
GGGCCCCCCGGG
ApaI (371) 1 site
GGGCCCCCCGGG
ApaI can be used between 25°C and 37°C.
Acc65I (373) 1 site
GGTACCCCATGG
KpnI (377) 1 site
GGTACCCCATGG
BssHII (410) 1 site
GCGCGCCGCGCG
BssHII is typically used at 50°C, but is 75% active at 37°C.
PvuII (595) 1 site
CAGCTGGTCGAC
BspQI (655) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different BspQI sites may not be compatible.
SapI (655) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII (771) 1 site
ACRYGTTGYRCA
Sticky ends from different AflIII sites may not be compatible.
PciI (771) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
NspI (775) 1 site
RCATGYYGTACR
AlwNI (1187) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
AhdI (1664) 1 site
GACNNNNNGTCCTGNNNNNCAG
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
BpmI (1734) 1 site
CTGGAG(N)14NNGACCTC(N)14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
NmeAIII (1812) 1 site
GCCGAG(N)18-19NNCGGCTC(N)18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM).
CMV IE94 promoter 3111 .. 4095 = 985 bp enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
CMV IE94 promoter 3111 .. 4095 = 985 bp enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
AmpR 1591 .. 2451 = 861 bp 286 amino acids = 31.6 kDa Segment 2: 1591 .. 2382 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics