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pCS2+

Vector that allows high-level transient expression in vertebrate cells as well as in vitro transcription/translation.

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pCS2+.dna
Map and Sequence File:    Download    Open   
Sequence Author:  I.M.A.G.E. Consortium
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HindIII (28) NdeI (3661) MscI * (3474) BseRI (3353) SalI (3108) NaeI (2909) NgoMIV (2907) DraIII (2806) XmnI (2263) BamHI (79) BspDI - ClaI (87) BstBI (92) EcoRI (94) StuI (103) AvaI - BsoBI - PaeR7I - XhoI (107) BmeT110I (108) XbaI (115) SnaBI (140) HpaI (278) MfeI (287) EagI - NotI (346) BtgI (349) SacII (352) BstXI - NsiI (364) PspOMI (367) ApaI (371) Acc65I (373) KpnI (377) BssHII (410) PvuII (595) BspQI - SapI (655) AflIII - PciI (771) NspI (775) AlwNI (1187) AhdI (1664) BpmI (1734) NmeAIII (1812) pCS2+ 4095 bp
HindIII  (28)
1 site		 A A G C T T T T C G A A
NdeI  (3661)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI  (3474)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
BseRI  (3353)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SalI  (3108)
1 site		 G T C G A C C A G C T G
NaeI  (2909)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2907)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (2806)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (2263)
1 site		 G A A N N N N T T C C T T N N N N A A G
BamHI  (79)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BspDI  (87)
1 site		 A T C G A T T A G C T A
ClaI  (87)
1 site		 A T C G A T T A G C T A
BstBI  (92)
1 site		 T T C G A A A A G C T T
EcoRI  (94)
1 site		 G A A T T C C T T A A G
StuI  (103)
1 site		 A G G C C T T C C G G A
AvaI  (107)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (107)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (107)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (107)
1 site		 C T C G A G G A G C T C
BmeT110I  (108)
1 site		 C Y C G R G G R G C Y C
XbaI  (115)
1 site		 T C T A G A A G A T C T
SnaBI  (140)
1 site		 T A C G T A A T G C A T
HpaI  (278)
1 site		 G T T A A C C A A T T G
MfeI  (287)
1 site		 C A A T T G G T T A A C
EagI  (346)
1 site		 C G G C C G G C C G G C
NotI  (346)
1 site		 G C G G C C G C C G C C G G C G
BtgI  (349)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
SacII  (352)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (364)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
NsiI  (364)
1 site		 A T G C A T T A C G T A
PspOMI  (367)
1 site		 G G G C C C C C C G G G
ApaI  (371)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
Acc65I  (373)
1 site		 G G T A C C C C A T G G
KpnI  (377)
1 site		 G G T A C C C C A T G G
BssHII  (410)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
PvuII  (595)
1 site		 C A G C T G G T C G A C
BspQI  (655)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (655)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (771)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (771)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
NspI  (775)
1 site		 R C A T G Y Y G T A C R
AlwNI  (1187)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (1664)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (1734)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (1812)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
CMV IE94 promoter
3111 .. 4095  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
CMV IE94 promoter
3111 .. 4095  =  985 bp
enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94
AmpR
1591 .. 2451  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1591 .. 2382  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1591 .. 2451  =  861 bp
286 amino acids  =  31.6 kDa