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pACEBac1

Baculovirus transfer vector with a polyhedrin promoter and a gentamycin resistance marker. Serves as an acceptor vector in the MultiBac™ system.

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pACEBac1 Sequence and MappACEBac1.dna
Map and Sequence File   
Sequence Author:  Geneva Biotech
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 BstXI (2734) BstZ17I (2723) AvrII (2715) BlpI (2693) BclI * (2619) PsiI (2503) HpaI (2483) MfeI (2470) ScaI (2370) HindIII (2355) PstI (2346) XbaI (2334) BstBI (2329) NotI (2320) BanII - SacI (2310) Eco53kI (2308) SalI - SgrDI (2300) StuI (2294) EcoRI (2284) BssHII (2277) RsrII (2269) BamHI (2262) AseI (2127) NruI (2109) BspDI - ClaI (2102) SpeI (2095) PmeI (2090) I-CeuI (2078) Bsu36I (2075) DraIII (1984) BsrGI (1811) PflFI - Tth111I (1629) BsmBI (1583) BtgZI (1519) AgeI - BsrFI (1) BspHI (114) AlwNI (425) ApaLI (520) BaeGI - Bme1580I (524) BseYI (530) PspFI (534) BciVI (636) BssS α I (661) BspQI - SapI (881) FspI (890) MscI (1111) SacII (1169) EcoRV (1226) BsaAI (1307) BseRI (1362) BsrDI (1376) pACEBac1 2904 bp
BstXI  (2734)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BstZ17I  (2723)
1 site
G T A T A C C A T A T G
AvrII  (2715)
1 site
C C T A G G G G A T C C
BlpI  (2693)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BclI  (2619)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PsiI  (2503)
1 site
T T A T A A A A T A T T
HpaI  (2483)
1 site
G T T A A C C A A T T G
MfeI  (2470)
1 site
C A A T T G G T T A A C
ScaI  (2370)
1 site
A G T A C T T C A T G A
HindIII  (2355)
1 site
A A G C T T T T C G A A
PstI  (2346)
1 site
C T G C A G G A C G T C
XbaI  (2334)
1 site
T C T A G A A G A T C T
BstBI  (2329)
1 site
T T C G A A A A G C T T
NotI  (2320)
1 site
G C G G C C G C C G C C G G C G
BanII  (2310)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (2310)
1 site
G A G C T C C T C G A G
Eco53kI  (2308)
1 site
G A G C T C C T C G A G
SalI  (2300)
1 site
G T C G A C C A G C T G
SgrDI  (2300)
1 site
C G T C G A C G G C A G C T G C
StuI  (2294)
1 site
A G G C C T T C C G G A
EcoRI  (2284)
1 site
G A A T T C C T T A A G
BssHII  (2277)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (2269)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BamHI  (2262)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
AseI  (2127)
1 site
A T T A A T T A A T T A
NruI  (2109)
1 site
T C G C G A A G C G C T
BspDI  (2102)
1 site
A T C G A T T A G C T A
ClaI  (2102)
1 site
A T C G A T T A G C T A
SpeI  (2095)
1 site
A C T A G T T G A T C A
PmeI  (2090)
1 site
G T T T A A A C C A A A T T T G
I-CeuI  (2078)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
Bsu36I  (2075)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
DraIII  (1984)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsrGI  (1811)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PflFI  (1629)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1629)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsmBI  (1583)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BtgZI  (1519)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AgeI  (1)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsrFI  (1)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BspHI  (114)
1 site
T C A T G A A G T A C T
AlwNI  (425)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (520)
1 site
G T G C A C C A C G T G
BaeGI  (524)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (524)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BseYI  (530)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (534)
1 site
C C C A G C G G G T C G
BciVI  (636)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSαI  (661)
1 site
C A C G A G G T G C T C
BspQI  (881)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (881)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
FspI  (890)
1 site
T G C G C A A C G C G T
MscI  (1111)
1 site
T G G C C A A C C G G T
SacII  (1169)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
EcoRV  (1226)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BsaAI  (1307)
1 site
Y A C G T R R T G C A Y
BseRI  (1362)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BsrDI  (1376)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7L
2739 .. 2904  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
Tn7L
2739 .. 2904  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
SV40 poly(A) signal
2484 .. 2618  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2484 .. 2618  =  135 bp
SV40 polyadenylation signal
MCS
2262 .. 2373  =  112 bp
multiple cloning site
MCS
2262 .. 2373  =  112 bp
multiple cloning site
polyhedrin promoter
2134 .. 2225  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
2134 .. 2225  =  92 bp
promoter for the baculovirus polyhedrin gene
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
Pc promoter
1925 .. 1953  =  29 bp
   Segment 3:  -10  
   1925 .. 1930  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
   Segment 2:  
   1931 .. 1947  =  17 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
   Segment 1:  -35  
   1948 .. 1953  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
3 segments
class 1 integron promoter
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