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Plasmid Files

pIDC

Baculovirus transfer vector with a polyhedrin promoter and a chloramphenicol resistance marker. Serves as a donor vector in the MultiBac™ system.

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pIDC Sequence and MappIDC.dna
Map and Sequence File   
Sequence Author:  Geneva Biotech
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 NruI (2306) BamHI (2149) RsrII (2143) BssHII (2134) StuI (2121) AccI (2112) SalI - SgrDI (2111) BanII - BsiHKAI - SacI (2109) Eco53kI (2107) XbaI (2077) PstI (2073) SfcI (2069) MfeI (1941) HpaI (1932) SmlI (1783) BpuEI (1768) BlpI (1719) AvrII (1696) PI-SceI (1671) BglII (1655) MslI (1593) MmeI (1583) BsrDI (1292) AclI (1220) BpmI - Eco57MI (1189) BspDI - ClaI (2311) BstXI (22) Acc65I (64) KpnI (68) SacII (71) SnaBI (119) BstAPI (513) AflIII (531) BseYI (582) PspFI (586) Bsu36I (651) AgeI - BsrFI (700) PflFI - Tth111I (760) BaeGI - Bme1580I (842) NcoI (1004) MscI (1042) PasI (1074) TaqII (1087) pIDC 2312 bp
NruI  (2306)
1 site
T C G C G A A G C G C T
BamHI  (2149)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
RsrII  (2143)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (2134)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
StuI  (2121)
1 site
A G G C C T T C C G G A
AccI  (2112)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2111)
1 site
G T C G A C C A G C T G
SgrDI  (2111)
1 site
C G T C G A C G G C A G C T G C
BanII  (2109)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (2109)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
SacI  (2109)
1 site
G A G C T C C T C G A G
Eco53kI  (2107)
1 site
G A G C T C C T C G A G
XbaI  (2077)
1 site
T C T A G A A G A T C T
PstI  (2073)
1 site
C T G C A G G A C G T C
SfcI  (2069)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
MfeI  (1941)
1 site
C A A T T G G T T A A C
HpaI  (1932)
1 site
G T T A A C C A A T T G
SmlI  (1783)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (1768)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BlpI  (1719)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
AvrII  (1696)
1 site
C C T A G G G G A T C C
PI-SceI  (1671)
1 site
A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C

PI-SceI  is a homing endonuclease that can recognize a variety of
similar recognition sequences.
BglII  (1655)
1 site
A G A T C T T C T A G A
MslI  (1593)
1 site
C A Y N N N N R T G G T R N N N N Y A C
MmeI  (1583)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrDI  (1292)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
AclI  (1220)
1 site
A A C G T T T T G C A A
BpmI  (1189)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (1189)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (2311)
1 site
A T C G A T T A G C T A
ClaI  (2311)
1 site
A T C G A T T A G C T A
BstXI  (22)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
Acc65I  (64)
1 site
G G T A C C C C A T G G
KpnI  (68)
1 site
G G T A C C C C A T G G
SacII  (71)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
SnaBI  (119)
1 site
T A C G T A A T G C A T
BstAPI  (513)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AflIII  (531)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BseYI  (582)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (586)
1 site
C C C A G C G G G T C G
Bsu36I  (651)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AgeI  (700)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsrFI  (700)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
PflFI  (760)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (760)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BaeGI  (842)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (842)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
NcoI  (1004)
1 site
C C A T G G G G T A C C
MscI  (1042)
1 site
T G G C C A A C C G G T
PasI  (1074)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TaqII  (1087)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
CmR
864 .. 1523  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
864 .. 1523  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
R6K γ ori
116 .. 472  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
R6K γ ori
116 .. 472  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
SV40 poly(A) signal
1798 .. 1932  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1798 .. 1932  =  135 bp
SV40 polyadenylation signal
cat promoter
1524 .. 1626  =  103 bp
promoter of the E. coli cat gene
cat promoter
1524 .. 1626  =  103 bp
promoter of the E. coli cat gene
polyhedrin promoter
2191 .. 2282  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
2191 .. 2282  =  92 bp
promoter for the baculovirus polyhedrin gene
MCS
2069 .. 2154  =  86 bp
multiple cloning site
MCS
2069 .. 2154  =  86 bp
multiple cloning site
loxP
30 .. 63  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
30 .. 63  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
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