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Plasmid Files

pGL2-Control

Control vector with the SV40 enhancer and promoter driving strong luciferase expression.

To see this sequence with restriction sites, features, and translations, please download
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pGL2-Control.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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GLprimer1 (6014 .. 6036) DraIII (5489) XmnI (4936) ScaI (4817) EaeI (4725) BsaI (4398) AhdI (4337) AlwNI (3860) PciI (3444) AfeI (3320) RVprimer4 (3245 .. 3264) PshAI (3259) AccI (3195) SalI (3194) BamHI (3188) SexAI * (3079) TspMI - XmaI (1) SmaI (3) Acc65I (8) KpnI (12) Eco53kI (16) SacI (18) MluI (22) NheI (28) BmtI (32) PaeR7I - XhoI (33) BglII (37) SfiI (176) StuI (222) AvrII (223) HindIII (239) GLprimer2 (269 .. 291) KasI (300) NarI (301) SfoI (302) PluTI (304) XbaI (315) PfoI * (375) BsiWI (422) BsrGI (758) EcoRI (855) BstEII (875) Bsu36I (881) XcmI (1003) EcoO109I - PpuMI (1447) PacI (1592) EcoRV (1606) BspDI - ClaI (1633) BfuAI - BspMI (1666) SgrAI (1696) EcoNI (1885) PflMI (2236) BsgI (2468) pGL2-Control 6047 bp
DraIII  (5489)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (4936)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (4817)
1 site
A G T A C T T C A T G A
EaeI  (4725)
1 site
Y G G C C R R C C G G Y
BsaI  (4398)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4337)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3860)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (3444)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AfeI  (3320)
1 site
A G C G C T T C G C G A
PshAI  (3259)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AccI  (3195)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (3194)
1 site
G T C G A C C A G C T G
BamHI  (3188)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SexAI  (3079)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
TspMI  (1)
1 site
C C C G G G G G G C C C
XmaI  (1)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (3)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Acc65I  (8)
1 site
G G T A C C C C A T G G
KpnI  (12)
1 site
G G T A C C C C A T G G
Eco53kI  (16)
1 site
G A G C T C C T C G A G
SacI  (18)
1 site
G A G C T C C T C G A G
MluI  (22)
1 site
A C G C G T T G C G C A
NheI  (28)
1 site
G C T A G C C G A T C G
BmtI  (32)
1 site
G C T A G C C G A T C G
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (33)
1 site
C T C G A G G A G C T C
BglII  (37)
1 site
A G A T C T T C T A G A
SfiI  (176)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (222)
1 site
A G G C C T T C C G G A
AvrII  (223)
1 site
C C T A G G G G A T C C
HindIII  (239)
1 site
A A G C T T T T C G A A
KasI  (300)
1 site
G G C G C C C C G C G G
NarI  (301)
1 site
G G C G C C C C G C G G