pGL3-Control

Control vector with the SV40 enhancer and promoter driving strong luciferase expression.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
KpnI (5) Acc65I (1) BsgI (5247) pause site RVprimer3 (5198 .. 5217) poly(A) signal NotI (5089) DraIII (4743) BsaAI (4740) XmnI (4190) AseI (3763) BsaI (3652) AhdI (3591) AlwNI (3114) PciI (2698) Eco53kI (9) SacI (11) MluI (15) MCS NheI (21) BmtI (25) TspMI - XmaI (26) SmaI - SrfI (28) PaeR7I - XhoI (32) BglII (36) SfiI (182) StuI (228) AvrII (229) HindIII (245) NcoI (278) GLprimer2 (281 .. 303) KasI (312) NarI (313) SfoI (314) PluTI (316) PfoI * (387) BstBI (449) BsrGI (770) BclI * (860) XcmI (1015) EcoO109I - PpuMI (1459) SgrAI (1708) XbaI (1934) FseI (1953) HpaI (2094) CsiI - SexAI * (2344) BamHI (2442) SalI (2448) AccI (2449) PshAI (2513) RVprimer4 (2499 .. 2518) AfeI (2574) pGL3-Control 5256 bp
KpnI  (5)
1 site
G G T A C C C C A T G G
Acc65I  (1)
1 site
G G T A C C C C A T G G
BsgI  (5247)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NotI  (5089)
1 site
G C G G C C G C C G C C G G C G
DraIII  (4743)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaAI  (4740)
1 site
Y A C G T R R T G C A Y
XmnI  (4190)
1 site
G A A N N N N T T C C T T N N N N A A G
AseI  (3763)
1 site
A T T A A T T A A T T A
BsaI  (3652)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3591)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3114)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (2698)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
Eco53kI  (9)
1 site
G A G C T C C T C G A G
SacI  (11)
1 site
G A G C T C C T C G A G
MluI  (15)
1 site
A C G C G T T G C G C A
NheI  (21)
1 site
G C T A G C C G A T C G
BmtI  (25)
1 site
G C T A G C C G A T C G
TspMI  (26)
1 site
C C C G G G G G G C C C
XmaI  (26)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (28)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (28)
1 site
G C C C G G G C C G G G C C C G
PaeR7I  (32)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (32)
1 site
C T C G A G G A G C T C
BglII  (36)
1 site
A G A T C T T C T A G A
SfiI  (182)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (228)
1 site
A G G C C T T C C G G A
AvrII  (229)
1 site
C C T A G G G G A T C C
HindIII  (245)
1 site
A A G C T T T T C G A A
NcoI  (278)
1 site
C C A T G G G G T A C C
KasI  (312)
1 site
G G C G C C C C G C G G
NarI  (313)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (314)
1 site
G G C G C C C C G C G G
PluTI  (316)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
PfoI  (387)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BstBI  (449)
1 site
T T C G A A A A G C T T
BsrGI  (770)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BclI  (860)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (1015)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoO109I  (1459)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (1459)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SgrAI  (1708)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XbaI  (1934)
1 site
T C T A G A A G A T C T
FseI  (1953)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
HpaI  (2094)
1 site
G T T A A C C A A T T G
CsiI  (2344)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (2344)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BamHI  (2442)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (2448)
1 site
G T C G A C C A G C T G
AccI  (2449)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PshAI  (2513)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AfeI  (2574)
1 site
A G C G C T T C G C G A
RVprimer3
20-mer  /  50% GC
1 binding site
5198 .. 5217  =  20 annealed bases
Tm  =  54°C
GLprimer2
23-mer  /  39% GC
1 binding site
281 .. 303  =  23 annealed bases
Tm  =  57°C
RVprimer4
20-mer  /  65% GC
1 binding site
2499 .. 2518  =  20 annealed bases
Tm  =  62°C
luciferase
280 .. 1932  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
luciferase
280 .. 1932  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
AmpR
3518 .. 4378  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3518 .. 4309  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3518 .. 4378  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4310 .. 4378  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3518 .. 4378  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2759 .. 3347  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
2759 .. 3347  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
4510 .. 4965  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4510 .. 4965  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 enhancer
2212 .. 2441  =  230 bp
SV40 enhancer
2212 .. 2441  =  230 bp
SV40 promoter
48 .. 244  =  197 bp
SV40 early promoter
SV40 promoter
48 .. 244  =  197 bp
SV40 early promoter
SV40 poly(A) signal
1973 .. 2094  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1973 .. 2094  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4379 .. 4483  =  105 bp
AmpR promoter
4379 .. 4483  =  105 bp
pause site
5158 .. 5249  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
5158 .. 5249  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
poly(A) signal
5096 .. 5144  =  49 bp
synthetic polyadenylation signal
poly(A) signal
5096 .. 5144  =  49 bp
synthetic polyadenylation signal
MCS
1 .. 41  =  41 bp
multiple cloning site
MCS
1 .. 41  =  41 bp
multiple cloning site
ORF:  280 .. 1932  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  3648 .. 3914  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  160 .. 417  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  565 .. 792  =  228 bp
ORF:  75 amino acids  =  9.1 kDa
ORF:  3518 .. 4378  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pGL3-Control.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.