Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI (5939) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI (5939) 1 site
ACCTGC(N)4TGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI (5766) 1 site
CGTCTCNGCAGAGN(N)4
Sticky ends from different BsmBI sites may not be compatible.
SpeI (5753) 1 site
ACTAGTTGATCA
BstZ17I (5434) 1 site
GTATACCATATG
SacII (5318) 1 site
CCGCGGGGCGCC
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI (5294) 1 site
CGATCGGCTAGC
Bsu36I (5280) 1 site
CCTNAGGGGANTCC
Sticky ends from different Bsu36I sites may not be compatible.
BstEII (4849) 1 site
GGTNACCCCANTGG
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI (4846) 1 site
CCANNNNNNTGGGGTNNNNNNACC
Sticky ends from different BstXI sites may not be compatible.
PstI (4846) 1 site
CTGCAGGACGTC
NotI (4822) 1 site
GCGGCCGCCGCCGGCG
AlwNI (4418) 1 site
CAGNNNCTGGTCNNNGAC
Sticky ends from different AlwNI sites may not be compatible.
ApaLI (4316) 1 site
GTGCACCACGTG
BciVI (4205) 1 site
GTATCC(N)5NCATAGG(N)5
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible.
PciI (4002) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
SalI (3752) 1 site
GTCGACCAGCTG
BstBI (3738) 1 site
TTCGAAAAGCTT
PmeI (3662) 1 site
GTTTAAACCAAATTTG
BssHII (3637) 1 site
GCGCGCCGCGCG
BssHII is typically used at 50°C, but is 75% active at 37°C.
BglI (8) 2 sites
GCCNNNNNGGCCGGNNNNNCCG
Sticky ends from different BglI sites may not be compatible.
SfiI (8) 2 sites
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
Acc65I (14) 1 site
GGTACCCCATGG
KpnI (18) 1 site
GGTACCCCATGG
Eco53kI (23) 1 site
GAGCTCCTCGAG
SacI (25) 1 site
GAGCTCCTCGAG
NheI (27) 1 site
GCTAGCCGATCG
BmtI (31) 1 site
GCTAGCCGATCG
AbsI (33) 1 site
CCTCGAGGGGAGCTCC
PaeR7I (33) 1 site
CTCGAGGAGCTC
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI (33) 1 site
VCTCGAGBBGAGCTCV
XhoI (33) 1 site
CTCGAGGAGCTC
EcoRV (41) 1 site
GATATCCTATAG
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII (46) 1 site
AGATCTTCTAGA
BglI (59) 2 sites
GCCNNNNNGGCCGGNNNNNCCG
Sticky ends from different BglI sites may not be compatible.
SfiI (59) 2 sites
GGCCNNNNNGGCCCCGGNNNNNCCGG
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
HindIII (65) 1 site
AAGCTTTTCGAA
MreI (163) 1 site
CGCCGGCGGCGGCCGC
SgrAI (163) 1 site
CRCCGGYGGYGGCCRC
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsrGI (590) 1 site
TGTACAACATGT
BsrGI is typically used at 37°C, but is even more active at 60°C.
BbvCI (811) 1 site
CCTCAGCGGAGTCG
KasI (1047) 1 site
GGCGCCCCGCGG
NarI (1048) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI (1049) 1 site
GGCGCCCCGCGG
PluTI (1051) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BlpI (1054) 1 site
GCTNAGCCGANTCG
Sticky ends from different BlpI sites may not be compatible.