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Plasmid Files

pLightSwitch_Prom

Vector for measuring promoter activity with an enhanced and destabilized Renilla luciferase (RenSP).

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pLightSwitch_Prom.dna
Map and Sequence File:    Download    Open   
Sequence Author:  SwitchGear Genomics
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AarI (3626) Forward Sequencing Primer (3576 .. 3598) BsmBI - Esp3I (3453) AhlI - BcuI - SpeI (3440) BpvUI - Ple19I - PvuI (2981) AseI - PshBI - VspI (2783) NmeAIII (2759) AhdI - AspEI - BmeRI - DriI - Eam1105I (2611) BstEII - BstPI - Eco91I - EcoO65I - PspEI (2536) BstXI - PstI (2533) AleI - OliI (2531) CciNI - NotI (2509) BspHI - CciI - PagI - RcaI (2409) AlwNI - CaiI (2105) BciVI - BfuI (1892) SfiI (8) Acc65I - Asp718I (14) KpnI (18) Ecl136II - Eco53kI - EcoICRI (23) Psp124BI - SacI - SstI (25) MluI (29) AsuNHI - NheI (35) BmtI - BspOI (39) Cfr9I - TspMI - XmaI - XmaCI (40) SmaI - SrfI (42) PaeR7I - Sfr274I - SlaI - XhoI (46) BglII (50) HindIII (67) Bsp19I - NcoI (110) Reverse Sequencing Primer (184 .. 205) AdeI - DraIII (300) BbuI - PaeI - SphI (538) Eco32I - EcoRV (585) PfoI * (635) BbvCI (760) XcmI (761) PvuII (764) BsePI - BssHII - PauI - PteI (843) DraII - EcoO109I (957) EcoRI (1044) XbaI (1171) FseI - RigI (1190) AanI - PsiI (1311) BstHPI - HpaI - KspAI (1331) MfeI - MunI (1340) BamHI (1433) SalI (1439) AccI - FblI - XmiI (1440) BoxI - BstPAI - PshAI (1504) BspLU11I - PciI - PscI (1689) AasI - DrdI - DseDI (1797) pLightSwitch_Prom 3656 bp
AarI  (3626)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BsmBI  (3453)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
Esp3I  (3453)
1 site
C G T