pSF-CMV-FMDV-RLuc

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the foot-and-mouth disease virus IRES.

Sequence Author: Oxford Genetics

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
PmeI (5531) RsrII (5224) BsrDI (4941) PflFI - Tth111I (4826) PmeI (4483) SanDI (4417) AscI (4326) FseI (4180) NaeI (4178) NgoMIV (4176) SwaI (4074) BspHI (3905) AlwNI (3601) BssSI (3358) SwaI (3181) PacI (3055) 3' β-globin insulator PstI - SbfI (2923) AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) NotI (858) Eco53kI (879) SacI (881) EcoRI (885) NcoI (905) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) BsgI (940) BspDI - ClaI (977) EcoNI (1011) PspOMI (1226) ApaI (1230) PflMI (1308) AleI (1309) AhdI (1438) BstAPI (1661) AanI (1765) NheI (2407) BmtI (2411) stop codons AanI (2557) HpaI (2577) MfeI (2586) pSF-CMV-FMDV-RLuc 5650 bp
PmeI  (5531)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5224)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4941)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (4826)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4826)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4483)
2 sites
G T T T A A A C C A A A T T T G
SanDI  (4417)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (4326)
1 site
G G C G C G C C C C G C G C G G
FseI  (4180)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (4178)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4176)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (4074)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (3905)
1 site
T C A T G A A G T A C T
AlwNI  (3601)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSI  (3358)
1 site
C A C G A G G T G C T C
SwaI  (3181)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3055)
1 site
T T A A T T A A A A T T A A T T
PstI  (2923)
1 site
C T G C A G G A C G T C
SbfI  (2923)
1 site
C C T G C A G G G G A C G T C C
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
NotI  (858)
1 site
G C G G C C G C C G C C G G C G
Eco53kI  (879)
1 site
G A G C T C C T C G A G
SacI  (881)
1 site
G A G C T C C T C G A G
EcoRI  (885)
1 site
G A A T T C C T T A A G
NcoI  (905)
1 site
C C A T G G G G T A C C
EcoRV  (921)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (928)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (928)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (928)
1 site
V C T C G A G B B G A G C T C V
XhoI  (928)
1 site
C T C G A G G A G C T C
BsgI  (940)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (977)
1 site
A T C G A T T A G C T A
ClaI  (977)
1 site
A T C G A T T A G C T A
EcoNI  (1011)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PspOMI  (1226)
1 site
G G G C C C C C C G G G
ApaI  (1230)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PflMI  (1308)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (1309)
1 site
C A C N N N N G T G G T G N N N N C A C
AhdI  (1438)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstAPI  (1661)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AanI  (1765)
2 sites
T T A T A A A A T A T T
NheI  (2407)
1 site
G C T A G C C G A T C G
BmtI  (2411)
1 site
G C T A G C C G A T C G
AanI  (2557)
2 sites
T T A T A A A A T A T T
HpaI  (2577)
1 site
G T T A A C C A A T T G
MfeI  (2586)
1 site
C A A T T G G T T A A C
RLuc
1473 .. 2411  =  939 bp
312 amino acids  =  36.1 kDa
Product: Renilla luciferase
codon-optimized synthetic gene
RLuc
1473 .. 2411  =  939 bp
312 amino acids  =  36.1 kDa
Product: Renilla luciferase
codon-optimized synthetic gene
NeoR/KanR
4580 .. 5374  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4580 .. 5374  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
3246 .. 3834  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3246 .. 3834  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
1012 .. 1457  =  446 bp
internal ribosome entry site (IRES) of the foot-and-mouth disease virus (FMDV)
IRES
1012 .. 1457  =  446 bp
internal ribosome entry site (IRES) of the foot-and-mouth disease virus (FMDV)
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
rrnG terminator
2768 .. 2904  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
2768 .. 2904  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
5387 .. 5523  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
5387 .. 5523  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
SV40 poly(A) signal
2456 .. 2577  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2456 .. 2577  =  122 bp
SV40 polyadenylation signal
MCS
857 .. 942  =  86 bp
multiple cloning site
MCS
857 .. 942  =  86 bp
multiple cloning site
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2949 .. 3020  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2949 .. 3020  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
T7 terminator
2692 .. 2738  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2692 .. 2738  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
stop codons
961 .. 971  =  11 bp
stop codons in all three reading frames
stop codons
961 .. 971  =  11 bp
stop codons in all three reading frames
stop codons
2414 .. 2424  =  11 bp
stop codons in all three reading frames
stop codons
2414 .. 2424  =  11 bp
stop codons in all three reading frames
RBS
4567 .. 4572  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
4567 .. 4572  =  6 bp
Shine-Dalgarno ribosome binding site
Kozak sequence
903 .. 909  =  7 bp
Kozak sequence
903 .. 909  =  7 bp
RBS
893 .. 898  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
893 .. 898  =  6 bp
Shine-Dalgarno ribosome binding site
ATG
907 .. 909  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
907 .. 909  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ORF:  4580 .. 5374  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  1473 .. 2411  =  939 bp
ORF:  312 amino acids  =  36.1 kDa
ORF:  2793 .. 3224  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  4752 .. 5138  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  5412 .. 196  =  435 bp
ORF:  144 amino acids  =  16.1 kDa
ORF:  1009 .. 1236  =  228 bp
ORF:  75 amino acids  =  8.4 kDa
ORF:  1389 .. 2327  =  939 bp
ORF:  312 amino acids  =  37.2 kDa
Click here to try SnapGene

Download pSF-CMV-FMDV-RLuc.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.