pCMV-LacZ

Plasmid for high-level expression of E. coli β-galactosidase in mammalian cells. Also known as pCMVβ, pCMVbeta, or pCMV-beta.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets
No matches
PluTI (7008) SfoI (7006) NarI (7005) KasI (7004) BstAPI (6954) XmnI (6381) ScaI (6262) BsaI (5843) AhdI (5782) BspQI - SapI (4773) HindIII (4530) SalI (4512) MfeI (4446) PsiI (4417) BlpI (3969) BsiWI (3727) EcoRI (1) CMV enhancer BsaAI - SnaBI (306) NcoI - StyI (326) BspEI (617) PaeR7I - PspXI - XhoI (640) PpuMI (702) BseRI (730) StuI (769) TspMI - XmaI (830) SmaI (832) Drosophila Adh Bsu36I (1180) Bpu10I (1461) BspDI - ClaI (1780) EcoRV (2069) DraIII (2146) BsaBI * (2287) BclI * (2302) BssHII - MauBI (2454) AfeI (2791) BstZ17I (3721) pCMV-LacZ 7164 bp
PluTI  (7008)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (7006)
1 site
G G C G C C C C G C G G
NarI  (7005)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7004)
1 site
G G C G C C C C G C G G
BstAPI  (6954)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
XmnI  (6381)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6262)
1 site
A G T A C T T C A T G A
BsaI  (5843)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5782)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (4773)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4773)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
HindIII  (4530)
1 site
A A G C T T T T C G A A
SalI  (4512)
1 site
G T C G A C C A G C T G
MfeI  (4446)
1 site
C A A T T G G T T A A C
PsiI  (4417)
1 site
T T A T A A A A T A T T
BlpI  (3969)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsiWI  (3727)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
EcoRI  (1)
1 site
G A A T T C C T T A A G
BsaAI  (306)
1 site
Y A C G T R R T G C A Y
SnaBI  (306)
1 site
T A C G T A A T G C A T
NcoI  (326)
1 site
C C A T G G G G T A C C
StyI  (326)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BspEI  (617)
1 site
T C C G G A A G G C C T
PaeR7I  (640)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (640)
1 site
V C T C G A G B B G A G C T C V
XhoI  (640)
1 site
C T C G A G G A G C T C
PpuMI  (702)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BseRI  (730)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (769)
1 site
A G G C C T T C C G G A
TspMI  (830)
1 site
C C C G G G G G G C C C
XmaI  (830)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (832)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Bsu36I  (1180)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
Bpu10I  (1461)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspDI  (1780)
1 site
A T C G A T T A G C T A
ClaI  (1780)
1 site
A T C G A T T A G C T A
EcoRV  (2069)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
DraIII  (2146)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaBI  (2287)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BclI  (2302)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BssHII  (2454)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (2454)
1 site
C G C G C G C G G C G C G C G C
AfeI  (2791)
1 site
A G C G C T T C G C G A
BstZ17I  (3721)
1 site
G T A T A C C A T A T G
Drosophila Adh
873 .. 965  =  93 bp
31 amino acids  =  3.3 kDa
Product: N-terminal fragment of Drosophila melanogaster alcohol dehydrogenase
Drosophila Adh
873 .. 965  =  93 bp
31 amino acids  =  3.3 kDa
Product: N-terminal fragment of Drosophila melanogaster alcohol dehydrogenase
lacZ
969 .. 4016  =  3048 bp
1015 amino acids  =  115.5 kDa
Product: β-galactosidase
lacZ
969 .. 4016  =  3048 bp
1015 amino acids  =  115.5 kDa
Product: β-galactosidase
AmpR
5709 .. 6569  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5709 .. 6500  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5709 .. 6569  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6501 .. 6569  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5709 .. 6569  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4950 .. 5538  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4950 .. 5538  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 330  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
331 .. 534  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
4303 .. 4437  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4303 .. 4437  =  135 bp
SV40 polyadenylation signal
AmpR promoter
6570 .. 6674  =  105 bp
AmpR promoter
6570 .. 6674  =  105 bp
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
SV40 intron
672 .. 768  =  97 bp
modified SV40 intron with splice donor and acceptor sites
ORF:  5839 .. 6105  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6955 .. 36  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  3890 .. 4174  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  873 .. 4016  =  3144 bp
ORF:  1047 amino acids  =  118.9 kDa
ORF:  4068 .. 4328  =  261 bp
ORF:  86 amino acids  =  10.3 kDa
ORF:  6915 .. 113  =  363 bp
ORF:  120 amino acids  =  13.4 kDa
ORF:  1815 .. 2129  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  3240 .. 3476  =  237 bp
ORF:  78 amino acids  =  9.0 kDa
ORF:  5709 .. 6569  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2879 .. 3190  =  312 bp
ORF:  103 amino acids  =  10.4 kDa
Click here to try SnapGene

Download pCMV-LacZ.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.