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Plasmid Files

pCMV6-AC-RFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to a C-terminal TurboRFP tag.

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pCMV6-AC-RFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6523) XmnI (5845) AlwNI (4772) PciI (4356) BstZ17I (3977) BsmI (3925) AanI (3864) PfoI (3780) BstBI (3687) PflFI - Tth111I (3123) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AsiSI - SgfI (1024) MreI - SgrAI (1026) AscI (1030) HindIII (1044) RsrII (1061) MluI (1067) BsiWI (1070) NotI (1076) PaeR7I - XhoI (1082) PasI (1306) Bsu36I (1341) PshAI (1715) PmeI (1787) FseI (1797) SacII (1801) AleI (1887) XL39 (reverse primer) (1893 .. 1912) EcoO109I (2017) BbvCI - Bpu10I (2113) BlpI (2157) BsmBI (2177) BstXI (2264) AgeI (2375) XcmI (2470) SexAI * (2562) StuI (2794) AvrII (2795) BsaBI * (2864) KasI (3004) NarI (3005) SfoI (3006) PluTI (3008) PstI (3058) pCMV6-AC-RFP 6595 bp
AanI  (6523)
2 sites
T T A T A A A A T A T T
XmnI  (5845)
1 site
G A A N N N N T T C C T T N N N N A A G
AlwNI  (4772)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4356)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3977)
1 site
G T A T A C C A T A T G
BsmI  (3925)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3864)
2 sites
T T A T A A A A T A T T
PfoI  (3780)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3687)
1 site
T T C G A A A A G C T T
PflFI  (3123)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3123)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A