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pCMV6-AN-FP602

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TurboFP602 tag.

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pCMV6-AN-FP602.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6550) XmnI (5872) AlwNI (4796) PciI (4380) BstZ17I (4001) BsmI (3949) AanI (3888) PfoI (3804) BstBI (3711) PflFI - Tth111I (3147) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) PasI (1250) Bsu36I (1285) PshAI (1659) BspEI (1725) AsiSI - SgfI (1741) MreI - SgrAI (1743) AscI (1747) HindIII (1761) RsrII (1778) MluI (1784) NotI (1794) PaeR7I - PspXI - XhoI (1802) PmeI (1811) FseI (1821) SacII (1825) XL39 (reverse primer) (1917 .. 1936) EcoO109I (2041) BbvCI - Bpu10I (2137) BlpI (2181) BsmBI (2201) AgeI (2399) XcmI (2494) SexAI * (2586) StuI (2818) AvrII (2819) BsaBI * (2888) KasI (3028) NarI (3029) SfoI (3030) PluTI (3032) PstI (3082) pCMV6-AN-FP602 6622 bp
AanI  (6550)
2 sites
T T A T A A A A T A T T
XmnI  (5872)
1 site
G A A N N N N T T C C T T N N N N A A G
AlwNI  (4796)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4380)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
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