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Plasmid Files

pCMV6-AN-GFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TurboGFP tag.

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pCMV6-AN-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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DraIII (6404) ScaI (5732) AlwNI (4778) PciI (4362) BstZ17I (3983) BsmI (3931) AanI (3870) PfoI (3786) BstBI (3693) AanI (6529) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) AarI (1471) PspOMI (1604) ApaI (1608) AsiSI - SgfI (1723) MreI - SgrAI (1725) AscI (1729) HindIII (1743) RsrII (1760) MluI (1766) NotI (1776) PaeR7I - PspXI - XhoI (1784) PmeI (1793) SacII (1807) AleI (1893) XL39 (reverse primer) (1899 .. 1918) BbsI (2016) BbvCI - Bpu10I (2119) BsmBI (2183) BstXI (2270) AgeI (2381) XcmI (2476) SexAI * (2568) SfiI (2754) StuI (2800) AvrII (2801) BclI * (2852) PflFI - Tth111I (3129) pCMV6-AN-GFP 6601 bp
DraIII  (6404)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (5732)
1 site
A G T A C T T C A T G A
AlwNI  (4778)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PciI  (4362)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (3983)
1 site
G T A T A C C A T A T G
BsmI  (3931)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3870)
2 sites
T T A T A A A A T A T T
PfoI  (3786)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3693)
1 site
T T C G A A A A G C T T
AanI  (6529)
2 sites
T T A T A A A A T A T T
BsrGI  (301)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (592)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (698)
1 site
T A C G T A A T G C A T
Eco53kI  (924)
1 site
G A G C T C C T C G A G
SacI  (926)
1 site
G A G C T C C T C G A G
EcoRI  (979)
1 site
G A A T T C C T T A A G
BamHI  (992)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Acc65I  (998)
1 site
G G