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Plasmid Files

pCMV6-AN-mYFP

PrecisionShuttle™ mammalian expression vector for fusing an ORF to an N-terminal TagYFP tag.

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pCMV6-AN-mYFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AanI (6562) ScaI (5765) PciI (4392) BstZ17I (4013) BsmI (3961) AanI (3900) BstBI (3723) PflFI - Tth111I (3159) BsrGI (301) NdeI (592) SnaBI (698) VP1.5 (forward primer) (839 .. 856) Eco53kI (924) SacI (926) EcoRI (979) BamHI (992) Acc65I (998) KpnI (1002) BmgBI (1085) BstEII (1202) PasI (1583) BspEI (1737) AsiSI - SgfI (1753) MreI - SgrAI (1755) AscI (1759) HindIII (1773) RsrII (1790) MluI (1796) NotI (1806) PaeR7I - PspXI - XhoI (1814) PmeI (1823) FseI (1833) XL39 (reverse primer) (1929 .. 1948) BbsI (2046) EcoO109I (2053) BbvCI (2149) BlpI (2193) BsmBI (2213) BstXI (2300) AgeI (2411) SexAI * (2598) SfiI (2784) StuI (2830) AvrII (2831) BclI * (2882) KasI (3040) NarI (3041) SfoI (3042) PluTI (3044) pCMV6-AN-mYFP 6634 bp
AanI  (6562)
2 sites
T T A T A A A A T A T T
ScaI  (5765)
1 site
A G T A C T T C A T G A
PciI  (4392)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (4013)
1 site
G T A T A C C A T A T G
BsmI  (3961)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
AanI  (3900)
2 sites
T T A T A A A A T A T T
BstBI  (3723)
1 site
T T C G A A A A G C T T
PflFI  (3159)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3159)
1 site
G A C N N N G T C C T G