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pFC27A HaloTag® CMV-neo

Flexi® vector with ampicillin and G418 resistance markers, for mammalian expression of a protein with a cleavable C-terminal HaloTag®.

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pFC27A HaloTag CMV-neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Promega
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BglII (6519) MluI (6400) BspEI (6187) PciI (5423) AgeI (5355) XmnI (4653) BstXI (4148) RsrII (3943) NaeI (3929) NgoMIV (3927) PluTI (3430) SfoI (3428) NarI (3427) KasI (3426) BsrGI (96) SpeI (152) NdeI (387) NheI (1052) BmtI (1056) AsiSI - PvuI - SgfI (1060) Eco53kI - EcoICRI (1444) SacI (1446) PaeR7I - XhoI (1447) PflMI (1533) AleI (1591) BstEII (1609) BmgBI (1743) PasI (1773) BclI * (1973) PshAI (2038) SgrAI (2125) EcoRI (2388) XbaI (2392) SalI (2398) SbfI (2408) BlpI (2473) NotI (2503) AanI - PsiI (2626) HpaI (2646) MfeI (2655) Acc65I (2883) KpnI (2887) SexAI * (3000) SfiI (3186) AvrII (3233) pFC27A HaloTag® CMV-neo 6524 bp
BglII  (6519)
1 site
A G A T C T T C T A G A
MluI  (6400)
1 site
A C G C G T T G C G C A
BspEI  (6187)
1 site
T C C G G A A G G C C T
PciI  (5423)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AgeI  (5355)
1 site
A C C G G T T G G C C A
XmnI  (4653)
1 site
G A A N N N N T T C C T T N N N N A A G
BstXI  (4148)
1 site
C C A N N N N N N T G G G G T