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Plasmid Files

pIRES

IRES-containing vector for expressing two genes in mammalian cells from the same bicistronic transcript.

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pIRES.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BglII (6100) AlwNI (5679) AhdI (5200) BpmI (5131) PfoI (4161) BstXI (3945) BstBI (3906) RsrII (3740) BssHII (3621) PflFI - Tth111I (3342) PluTI (3227) SfoI (3225) NarI (3224) KasI (3223) BsrGI (96) SpeI (152) NdeI (387) SnaBI (493) AsiSI (664) Eco53kI (727) SacI (729) I-PpoI (851) NheI (1085) BmtI (1089) PaeR7I - XhoI (1091) EcoRI (1096) MluI (1102) PspOMI (1265) ApaI (1269) PmlI (1468) AarI (1491) PflMI (1605) PciI (1642) BmgBI (1695) XbaI (1747) SalI (1753) AccI (1754) NotI (1764) HpaI (1930) MfeI (1939) SexAI * (2797) SfiI (2983) StuI (3029) pIRES 6105 bp
BglII  (6100)
1 site
A G A T C T T C T A G A
AlwNI  (5679)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (5200)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (5131)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PfoI  (4161)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstXI  (3945)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BstBI  (3906)
1 site
T T C G A A A A G C T T
RsrII  (3740)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (3621)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI  (3342)
1 site
G A C N N N G T C C