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Plasmid Files

pIRES2-EGFP

IRES-containing bicistronic vector for expressing a gene together with EGFP.

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pIRES2-EGFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BsaI (4321) RsrII (3848) PflFI - Tth111I (3450) FspI (3434) PluTI (3335) SfoI (3333) NarI (3332) KasI (3331) BspDI * - ClaI * (3172) StuI (3153) SfiI (3107) SexAI * (2921) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) EcoRI (629) PstI (638) SalI (639) AccI (640) SacII (652) TspMI - XmaI (656) SmaI (658) BamHI (660) AclI (690) XmnI (879) PmlI (983) AarI (1006) PflMI (1120) BmgBI (1210) BstXI (1253) BsrGI (1963) NotI (1976) XbaI * (1986) MfeI (2082) HpaI (2095) AflII (2214) pIRES2-EGFP 5308 bp
BsaI  (4321)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3848)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (3450)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3450)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (3434)
1 site
T G C G C A A C G C G T
PluTI  (3335)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3333)
1 site
G G C G C C C C G C G G
NarI  (3332)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3331)
1 site
G G C G C C C C G C G G
BspDI  (3172)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (3172)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (3153)
1 site
A G G C C T T C C G G A
SfiI  (3107)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SexAI  (2921)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
BglII  (609)
1 site
A G A