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Plasmid Files

pNTAP-C

Vector for expressing proteins N-terminally tagged with calmodulin- and streptavidin-binding peptides. For other reading frames, use pNTAP‑A or pNTAP‑B.

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pNTAP-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Agilent Technologies
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PciI (4480) ApaLI (4166) BsaI (3551) PfoI (3337) BstBI (3244) RsrII (3078) BsrDI (2795) PflFI - Tth111I (2680) MscI (2644) PluTI (2565) SfoI (2563) NarI (2562) KasI (2561) StuI (2383) BseRI (2380) NdeI (240) SnaBI (346) NheI (597) BmtI (601) Eco53kI (653) SacI (655) AleI (661) SacII (662) BstXI (663) NotI (668) BsmBI (717) BbsI (793) PmlI (813) SrfI (918) BamHI (923) PstI (939) EcoRI (941) EcoRV (949) HindIII (953) SalI (968) AccI (969) AbsI - PaeR7I - PspXI - XhoI (974) PspOMI (983) ApaI (987) PvuI (1065) BclI * (1219) MfeI (1312) HpaI (1325) Bts α I (1401) MluI (1448) SfiI (2337) pNTAP-C 4532 bp
PciI  (4480)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4166)
1 site
G T G C A C C A C G T G
BsaI  (3551)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3337)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (3244)
1 site
T T C G A A A A G C T T
RsrII  (3078)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2795)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2680)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2680)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (2644)
1 site
T G G C C A A C C G G T
PluTI  (2565)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2563)
1 site
G G C G C C C C G C G G
NarI  (2562)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2561)
1 site
G G C G C C C C G C G G
StuI  (2383)
1 site
A G G C C T T C C G G A
BseRI  (2380)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
NdeI  (240)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (346)
1 site
T A C G T A A T G C A T
NheI  (597)
1 site
G C T A G C C G A T C G
BmtI  (601)
1 site
G C T A G C C G A T C G
Eco53kI  (653)
1 site
G A G C T C C T C G A G
SacI  (655)
1 site
G A G C T C C T C G A G
AleI  (661)
1 site
C A C N N N N G T G G T G N N N N C A C
SacII  (662)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (663)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NotI  (668)
1 site
G C G G C C G C C G C C G G C G
BsmBI  (717)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BbsI  (793)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PmlI  (813)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
SrfI  (918)
1 site
G C C C G G G C C G G G C C C G
BamHI  (923)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PstI  (939)
1 site
C T G C A G G A C G T C
EcoRI  (941)
1 site
G A A T T C C T T A A G
EcoRV  (949)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII  (953)
1 site
A A G C T T T T C G A A
SalI  (968)
1 site
G T C G A C C A G C T G