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Plasmid Files

pPTuner

Vector for fusing a destabilization domain to the N-terminus of a partner protein.

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pPTuner.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4264) EcoO109I (3444) BsaI (3335) PfoI (3121) RsrII (2862) BsrDI (2579) PflFI - Tth111I (2464) FspI (2448) MscI (2428) PluTI (2349) SfoI (2347) NarI (2346) KasI (2345) EagI (2252) BspDI * - ClaI * (2186) StuI (2167) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) AarI (605) BsmBI (631) BsgI (633) BbsI (893) BglII (930) PaeR7I - XhoI (934) Eco53kI (939) SacI (941) HindIII (943) EcoRI (950) PstI (959) SalI (960) AccI (961) Acc65I (966) KpnI (970) SacII (973) PspOMI (974) ApaI (978) BamHI (981) XbaI * (993) BclI * (1003) MfeI (1096) HpaI (1109) Bts α I (1185) MluI (1232) DraIII (1462) SexAI * (1935) SfiI (2121) BseRI (2164) pPTuner 4322 bp
PciI  (4264)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3444)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3335)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3121)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2862)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2579)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2464)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2464)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2448)
1 site
T G C G C A A C G C G T
MscI  (2428)
1 site
T G G C C A A C C G G T
PluTI  (2349)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2347)
1 site
G G C G C C C C G C G G
NarI  (2346)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2345)
1 site
G G C G C C C C G C G G
EagI  (2252)
1 site
C G G C C G G C C G G C
BspDI  (2186)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2186)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2167)
1 site
A G G C C T T C C G G A
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
AarI  (605)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BsmBI  (631)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsgI  (633)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbsI  (893)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (930)
1 site
A G A T C T T C T A G A
PaeR7I  (934)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (934)
1 site
C T C G A G G A G C T C
Eco53kI  (939)
1 site
G A G C T C C T C G A G
SacI  (941)
1 site
G A G C T C C T C G A G
HindIII  (943)
1 site
A A G C T T T T C G A A
EcoRI  (950)
1 site
G A A T T C C T