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prHom-Sec1

Mammalian vector encoding four FKBP homodimerizer domains, for creating ER-localized oligomers that can be dissociated with a drug. Also known as pC4S1-FM4-FCS-hGH.

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prHom-Sec1 Sequence and MapprHom-Sec1.dna
Map and Sequence File   
Sequence Author:  Clontech
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 NaeI (6688) NgoMIV (6686) DraIII (6585) BsaI (5186) AhdI (5125) BspQI - SapI (4116) SfiI (3931) StuI (3882) AvrII (3877) AbsI - PaeR7I - PspXI - XhoI (3854) PflMI (3611) MluI (1) CMV enhancer SnaBI (298) EcoRI (656) XbaI (770) SpeI (2078) AflII (2119) PstI (2241) KasI (2414) NarI (2415) SfoI (2416) PluTI (2418) PflFI - Tth111I (2618) BamHI (2685) KflI (2714) HincII (2866) MfeI (3056) PspOMI (3231) ApaI (3235) MscI (3376) prHom-Sec1 7002 bp
NaeI  (6688)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (6686)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
DraIII  (6585)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaI  (5186)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5125)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspQI  (4116)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4116)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
SfiI  (3931)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (3882)
1 site
A G G C C T T C C G G A
AvrII  (3877)
1 site
C C T A G G G G A T C C
AbsI  (3854)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (3854)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3854)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3854)
1 site
C T C G A G G A G C T C
PflMI  (3611)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
MluI  (1)
1 site
A C G C G T T G C G C A
SnaBI  (298)
1 site
T A C G T A A T G C A T
EcoRI  (656)
1 site
G A A T T C C T T A A G
XbaI  (770)
1 site
T C T A G A A G A T C T
SpeI  (2078)
1 site
A C T A G T T G A T C A
AflII  (2119)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
PstI  (2241)
1 site
C T G C A G G A C G T C
KasI  (2414)
1 site
G G C G C C C C G C G G
NarI  (2415)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (2416)
1 site
G G C G C C C C G C G G
PluTI  (2418)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
PflFI  (2618)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2618)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BamHI  (2685)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
KflI  (2714)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
HincII  (2866)
1 site
G T Y R A C C A R Y T G
MfeI  (3056)
1 site
C A A T T G G T T A A C
PspOMI  (3231)
1 site
G G G C C C C C C G G G
ApaI  (3235)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
MscI  (3376)
1 site
T G G C C A A C C G G T
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 2:  
   5052 .. 5855  =  804 bp
   267 amino acids  =  29.4 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
   Segment 1:  signal sequence  
   5856 .. 5924  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5052 .. 5924  =  873 bp
290 amino acids  =  32.0 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4293 .. 4881  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
stuffer sequence
2105 .. 2677  =  573 bp
191 amino acids  =  22.1 kDa
to be replaced by the coding sequence of interest
stuffer sequence
2105 .. 2677  =  573 bp
191 amino acids  =  22.1 kDa
to be replaced by the coding sequence of interest
β-globin intron
2704 .. 3276  =  573 bp
intron from rabbit β-globin gene
β-globin intron
2704 .. 3276  =  573 bp
intron from rabbit β-globin gene
f1 ori
6361 .. 6816  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
6361 .. 6816  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
DmrD
776 .. 1096  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
776 .. 1096  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1103 .. 1423  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1103 .. 1423  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1430 .. 1750  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1430 .. 1750  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1757 .. 2077  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
DmrD
1757 .. 2077  =  321 bp
107 amino acids  =  11.8 kDa
Product: dimeric F36M mutant of FK506-binding
protein FKBP12
dimerization can be reversed by ligand binding
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
19 .. 322  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
323 .. 526  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
SV40 promoter
3861 .. 4056  =  196 bp
SV40 promoter
3861 .. 4056  =  196 bp
AmpR promoter
5925 .. 6029  =  105 bp
AmpR promoter
5925 .. 6029  =  105 bp
hGH signal sequence
692 .. 769  =  78 bp
26 amino acids  =  2.7 kDa
Product: human growth hormone signal sequence
hGH signal sequence
692 .. 769  =  78 bp
26 amino acids  =  2.7 kDa
Product: human growth hormone signal sequence
β-globin poly(A) signal
3473 .. 3528  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
3473 .. 3528  =  56 bp
rabbit β-globin polyadenylation signal
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus
thymidine kinase gene
HSV TK 5'-UTR
616 .. 655  =  40 bp
5' untranslated region from the herpes simplex virus
thymidine kinase gene
furin cleavage site
2087 .. 2104  =  18 bp
6 amino acids  =  857.0 Da
Product: cleavage site for the furin protease in the
trans-Golgi network
furin cleavage site
2087 .. 2104  =  18 bp
6 amino acids  =  857.0 Da
Product: cleavage site for the furin protease in the
trans-Golgi network
SV40 ori
3881 .. 4016  =  136 bp
SV40 origin of replication
SV40 ori
3881 .. 4016  =  136 bp
SV40 origin of replication
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