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Plasmid Files

pBI121

Binary Agrobacterium vector with a GUS reporter gene for plant transformation.

 
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pBI121.dna
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NotI (14,662) PasI (14,110) BstEII (13,001) BstXI (12,671) SfiI (11,038) PflMI * (10,709) NdeI (10,475) FspAI (9695) KflI (9097) BbvCI (9007) AhdI (8953) DraIII (8290) EcoRI (7982) SacI (7715) EcoNI (1627) PmeI (2492) Bsu36I (2700) NheI (2715) BmtI (2719) PspOMI (3899) ApaI (3903) BspDI - ClaI (4537) HindIII (4950) SbfI (4966) BsaI (5045) ScaI (5179) XbaI (5815) BamHI (5821) TspMI - XmaI (5826) SmaI (5828) SnaBI (6229) Eco53kI (7713) pBI121 14,758 bp
NotI  (14,662)
1 site
G C G G C C G C C G C C G G C G
PasI  (14,110)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BstEII  (13,001)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (12,671)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SfiI  (11,038)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PflMI  (10,709)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NdeI  (10,475)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
FspAI  (9695)
1 site
R T G C G C A Y Y A C G C G T R
KflI  (9097)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BbvCI  (9007)
1 site
C C T C A G C G G A G T C G
AhdI  (8953)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (8290)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
EcoRI  (7982)
1 site
G A A T T C C T T A A G
SacI  (7715)
1 site
G A G C T C C T C G A G
EcoNI  (1627)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PmeI  (2492)
1 site
G T T T A A A C C A A A T T T G
Bsu36I  (2700)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NheI  (2715)
1 site
G C T A G C C G A T C G
BmtI  (2719)
1 site
G C T A G C C G A T C G
PspOMI  (3899)
1 site
G G G C C C C C C G G G
ApaI  (3903)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BspDI  (4537)
1 site
A T C G A T T A G C T A
ClaI  (4537)
1 site
A T C G A T T A G C T A
HindIII  (4950)
1 site
A A G C T T T T C G A A
SbfI  (4966)
1 site
C C T G C A G G G G A C G T C C
BsaI  (5045)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (5179)
1 site
A G T A C T T C A T G A
XbaI  (5815)
1 site
T C T A G A A G A T C T
BamHI  (5821)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (5826)
1 site
C C C G G G G G G C C C
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