Plasmid Files

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PsiI  (11,415) 1 site

BspHI  (11,089) 1 site

EcoRV  (10,666) 1 site

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.

PluTI  (9407) 1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

SfoI  (9405) 1 site

NarI  (9404) 1 site

Efficient cleavage requires at least two copies of the NarI recognition sequence.

KasI  (9403) 1 site

EcoNI  (8931) 1 site

The 1-base overhangs produced by EcoNI may be hard to ligate. Sticky ends from different EcoNI sites may not be compatible.

BmtI  (8622) 1 site

NheI  (8618) 1 site

BsiWI  (8502) 1 site

BsiWI is typically used at 55°C, but is 50% active at 37°C.

PmeI  (5490) 1 site

AseI  (92) 1 site

Acc65I  (407) 1 site

KpnI  (411) 1 site

FspAI  (521) 1 site

AarI  (585) 1 site

Efficient cleavage requires at least two copies of the AarI recognition sequence. Sticky ends from different AarI sites may not be compatible. After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.

AfeI  (758) 1 site

BstBI  (1770) 1 site

StuI  (1868) 1 site

PshAI  (2467) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

PaeR7I  (2654) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

PspXI  (2654) 1 site

XhoI  (2654) 1 site

BspEI  (3215) 1 site

BbvCI  (3944) 1 site

SrfI  (4090) 1 site

BstXI  (4207) 1 site

Sticky ends from different BstXI sites may not be compatible.

AvrII  (4479) 1 site

PacI  (4526) 1 site

SpeI  (4538) 1 site

MfeI  (4842) 1 site

BstEII  (5114) 1 site

Sticky ends from different BstEII sites may not be compatible. BstEII is typically used at 60°C, but is 50% active at 37°C.

AhdI  (5244) 1 site

The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.

SbfI  (5269) 1 site

HindIII  (5277) 1 site

PvuI  (5403) 1 site

pVS1 RepA 7887 .. 8960  =  1074 bp 357 amino acids  =  39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1

pVS1 RepA 7887 .. 8960  =  1074 bp 357 amino acids  =  39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1

KanR 10,565 .. 11,359  =  795 bp 264 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

KanR 10,565 .. 11,359  =  795 bp 264 amino acids  =  31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin

OCS terminator 4554 .. 5261  =  708 bp octopine synthase terminator

OCS terminator 4554 .. 5261  =  708 bp octopine synthase terminator

CmR 3006 .. 3665  =  660 bp 219 amino acids  =  25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol

CmR 3006 .. 3665  =  660 bp 219 amino acids  =  25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol

pVS1 StaA 6829 .. 7458  =  630 bp 209 amino acids  =  22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1

pVS1 StaA 6829 .. 7458  =  630 bp 209 amino acids  =  22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1

ori 9890 .. 10,478  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 9890 .. 10,478  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

BlpR 373 .. 924  =  552 bp 183 amino acids  =  20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin

BlpR 373 .. 924  =  552 bp 183 amino acids  =  20.6 kDa Product: phosphinothricin acetyltransferase confers resistance to bialophos or phosphinothricin

MAS promoter 930 .. 1310  =  381 bp mannopine synthase promoter (Velten et al., 1984)

MAS promoter 930 .. 1310  =  381 bp mannopine synthase promoter (Velten et al., 1984)

CaMV 35S promoter 2306 .. 2651  =  346 bp strong constitutive promoter from cauliflower mosaic virus

CaMV 35S promoter 2306 .. 2651  =  346 bp strong constitutive promoter from cauliflower mosaic virus

ccdB 4007 .. 4312  =  306 bp 101 amino acids  =  11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.

ccdB 4007 .. 4312  =  306 bp 101 amino acids  =  11.7 kDa Product: CcdB, a bacterial toxin that poisons DNA gyrase Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.

MAS terminator 111 .. 363  =  253 bp mannopine synthase terminator

MAS terminator 111 .. 363  =  253 bp mannopine synthase terminator

pVS1 oriV 9026 .. 9220  =  195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

pVS1 oriV 9026 .. 9220  =  195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)

bom 9564 .. 9704  =  141 bp basis of mobility region from pBR322

bom 9564 .. 9704  =  141 bp basis of mobility region from pBR322

attR2 4353 .. 4477  =  125 bp recombination site for the Gateway® LR reaction

attR2 4353 .. 4477  =  125 bp recombination site for the Gateway® LR reaction

attR1 2773 .. 2892  =  120 bp recombination site for the Gateway® LR reaction

attR1 2773 .. 2892  =  120 bp recombination site for the Gateway® LR reaction

TMV Ω 2681 .. 2735  =  55 bp translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)

TMV Ω 2681 .. 2735  =  55 bp translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)

lac UV5 promoter 2922 .. 2952  =  31 bp    Segment 1:  -35      2922 .. 2927  =  6 bp E. coli lac promoter with an "up" mutation

lac UV5 promoter 2922 .. 2952  =  31 bp    Segment 2:      2928 .. 2945  =  18 bp E. coli lac promoter with an "up" mutation

lac UV5 promoter 2922 .. 2952  =  31 bp    Segment 3:  -10      2946 .. 2952  =  7 bp E. coli lac promoter with an "up" mutation

lac UV5 promoter 2922 .. 2952  =  31 bp 3 segments E. coli lac promoter with an "up" mutation

Myc 2740 .. 2769  =  30 bp 10 amino acids  =  1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag

Myc 2740 .. 2769  =  30 bp 10 amino acids  =  1.2 kDa Product: Myc (human c-Myc oncogene) epitope tag

LB T-DNA repeat 1 .. 25  =  25 bp left border repeat from nopaline C58 T-DNA

LB T-DNA repeat 1 .. 25  =  25 bp left border repeat from nopaline C58 T-DNA

RB T-DNA repeat 5505 .. 5529  =  25 bp right border repeat from nopaline C58 T-DNA

RB T-DNA repeat 5505 .. 5529  =  25 bp right border repeat from nopaline C58 T-DNA

ATG 2737 .. 2739  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 2737 .. 2739  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

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