EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PluTI (9407) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI (9405) 1 site
GGCGCCCCGCGG
NarI (9404) 1 site
GGCGCCCCGCGG
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI (9403) 1 site
GGCGCCCCGCGG
EcoNI (8931) 1 site
CCTNNNNNAGGGGANNNNNTCC
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible.
BmtI (8622) 1 site
GCTAGCCGATCG
NheI (8618) 1 site
GCTAGCCGATCG
BsiWI (8502) 1 site
CGTACGGCATGC
BsiWI is typically used at 55°C, but is 50% active at 37°C.
PmeI (5490) 1 site
GTTTAAACCAAATTTG
Acc65I (407) 1 site
GGTACCCCATGG
KpnI (411) 1 site
GGTACCCCATGG
FspAI (521) 1 site
RTGCGCAYYACGCGTR
AarI (585) 1 site
CACCTGC(N)4GTGGACG(N)4(N)4
Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.