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Plasmid Files

pQE-17

Bacterial vector for expressing DHFR fusion proteins. Similar to pQE‑18, but with a different reading frame after the DHFR.

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pQE-17.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qiagen
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BbsI (3990) EcoO109I (3988) AatII (3934) ZraI (3932) FspI (3234) NmeAIII (3160) BglI (3132) BsrFI (3092) AhdI (3012) PspFI (2427) BseYI (2423) PciI (2119) BspQI - SapI (2003) AvaI - BsoBI - PaeR7I - XhoI (1) BmeT110I (2) PsiI (49) MfeI (59) EcoRI (88) BamHI (121) SexAI * (301) Eco53kI (391) BanII - SacI (393) BstXI (411) AflII (423) EcoNI (634) BglII (697) HindIII (729) BlpI (741) NheI (849) BmtI (853) PvuII (1001) BspEI (1097) PasI (1333) MscI (1368) BtgI - NcoI - StyI (1402) XbaI (1706) PflFI - Tth111I (1866) BsaAI (1873) BstZ17I (1892) NdeI (1942) BstAPI (1943) pQE-17 4004 bp
BbsI  (3990)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3988)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3934)
1 site
G A C G T C C T G C A G
ZraI  (3932)
1 site
G A C G T C C T G C A G
FspI  (3234)
1 site
T G C G C A A C G C G T
NmeAIII  (3160)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (3132)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (3092)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3012)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (2427)
1 site
C C C A G C G G G T C G
BseYI  (2423)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (2119)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2003)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2003)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BamHI  (121)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SexAI  (301)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
Eco53kI  (391)
1 site
G A G