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Plasmid Files

pQE-51

Bacterial vector for expressing untagged proteins. For other reading frames, use pQE‑50 or pQE‑52.

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pQE-51 Sequence and MappQE-51.dna
Map and Sequence File   
Sequence Author:  Qiagen
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 BbsI (3423) EcoO109I (3421) AatII (3367) ZraI (3365) XmnI (3044) PvuI (2815) FspI (2667) AseI (2617) NmeAIII (2593) BglI (2565) BsrFI (2525) BsaI (2506) AhdI (2445) AlwNI (1968) PspFI (1860) BseYI (1856) PaeR7I - XhoI (1) T5 promoter PsiI (49) MfeI (59) lac operator EcoRI (88) BseRI (115) ATG BamHI (120) SphI (130) Eco53kI (134) BanII - SacI (136) Acc65I (138) KpnI (142) TspMI - XmaI (143) SmaI (145) SalI (148) HincII (150) PstI (158) BfuAI - BspMI (161) HindIII (162) BlpI (174) NheI (282) BmtI (286) Bpu10I (307) PvuII (434) BspEI (530) PasI - PflMI * (766) MscI (801) BtgI - NcoI - StyI (835) XbaI (1139) PfoI (1196) PflFI - Tth111I (1299) BsaAI (1306) BstZ17I (1325) NdeI (1375) BstAPI (1376) BspQI - SapI (1436) AflIII - PciI (1552) pQE-51 3437 bp
BbsI  (3423)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
EcoO109I  (3421)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (3367)
1 site
G A C G T C C T G C A G
ZraI  (3365)
1 site
G A C G T C C T G C A G
XmnI  (3044)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (2815)
1 site
C G A T C G G C T A G C
FspI  (2667)
1 site
T G C G C A A C G C G T
AseI  (2617)
1 site
A T T A A T T A A T T A
NmeAIII  (2593)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2565)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (2525)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BsaI  (2506)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2445)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (1968)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (1860)
1 site
C C C A G C G G G T C G
BseYI  (1856)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
EcoRI  (88)
1 site
G A A T T C C T T A A G
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BamHI  (120)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
SphI  (130)
1 site
G C A T G C C G T A C G
Eco53kI  (134)
1 site
G A G C T C C T C G A G
BanII  (136)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (136)
1 site
G A G C T C C T C G A G
Acc65I  (138)
1 site
G G T A C C C C A T G G
KpnI  (142)
1 site
G G T A C C C C A T G G
TspMI  (143)
1 site
C C C G G G G G G C C C
XmaI  (143)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (145)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SalI  (148)
1 site
G T C G A C C A G C T G
HincII  (150)
1 site
G T Y R A C C A R Y T G
PstI  (158)
1 site
C T G C A G G A C G T C
BfuAI  (161)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (161)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
HindIII  (162)
1 site
A A G C T T T T C G A A
BlpI  (174)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NheI  (282)
1 site
G C T A G C C G A T C G
BmtI  (286)
1 site
G C T A G C C G A T C G
Bpu10I  (307)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (434)
1 site
C A G C T G G T C G A C
BspEI  (530)
1 site
T C C G G A A G G C C T
PasI  (766)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (766)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
MscI  (801)
1 site
T G G C C A A C C G G T
BtgI  (835)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (835)
1 site
C C A T G G G G T A C C
StyI  (835)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
XbaI  (1139)
1 site
T C T A G A A G A T C T
PfoI  (1196)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (1299)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1299)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (1306)
1 site
Y A C G T R R T G C A Y
BstZ17I  (1325)
1 site
G T A T A C C A T A T G
NdeI  (1375)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
BstAPI  (1376)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BspQI  (1436)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1436)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
AflIII  (1552)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (1552)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AmpR
2372 .. 3232  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   2372 .. 3163  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2372 .. 3232  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   3164 .. 3232  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2372 .. 3232  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
321 .. 980  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
321 .. 980  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
1613 .. 2201  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1613 .. 2201  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
3233 .. 3337  =  105 bp
AmpR promoter
3233 .. 3337  =  105 bp
lambda t0 terminator
183 .. 277  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
183 .. 277  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
1045 .. 1131  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
1045 .. 1131  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
MCS
120 .. 167  =  48 bp
multiple cloning site
MCS
120 .. 167  =  48 bp
multiple cloning site
T5 promoter
10 .. 54  =  45 bp
   Segment 1:  
   10 .. 24  =  15 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
   Segment 2:  -35  
   25 .. 30  =  6 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
   Segment 3:  
   31 .. 47  =  17 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
   Segment 4:  -10  
   48 .. 54  =  7 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
101 .. 106  =  6 bp
ribosome binding site
RBS
101 .. 106  =  6 bp
ribosome binding site
ATG
115 .. 117  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
115 .. 117  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
stop codons
167 .. 177  =  11 bp
stop codons in all three reading frames
stop codons
167 .. 177  =  11 bp
stop codons in all three reading frames
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