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Plasmid Files

pLVX-Hom-1

Lentiviral vector encoding an FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug.

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pLVX-Hom-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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XcmI (8335) SgrDI (8110) SspI (7993) PvuI (7559) FspI (7411) AhdI (7189) DrdI (6407) BmtI (5915) NheI (5911) Bsu36I (5121) PstI (5005) MluI (4387) SexAI * (4354) NruI * (833) FseI (1150) EcoNI (1170) MfeI (1189) BbvCI - Bpu10I (1424) AleI (1577) KflI - PpuMI (1934) BspDI - ClaI (2180) NdeI (2374) EcoRI (2803) PaeR7I - XhoI (2809) SpeI (2815) XbaI (2827) NotI (3156) BamHI (3163) PspOMI (3294) ApaI (3298) AvrII (3332) BmgBI (3724) PflFI - Tth111I (3829) BsiWI (3843) RsrII (3903) BstEII (3921) MscI * (4162) pLVX-Hom-1 8478 bp
XcmI  (8335)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
SgrDI  (8110)
1 site
C G T C G A C G G C A G C T G C
SspI  (7993)
1 site
A A T A T T T T A T A A
PvuI  (7559)
1 site
C G A T C G G C T A G C
FspI  (7411)
1 site
T G C G C A A C G C G T
AhdI  (7189)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (6407)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BmtI  (5915)
1 site
G C T A G C C G A T C G
NheI  (5911)
1 site
G C T A G C C G A T C G
Bsu36I  (5121)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PstI  (5005)
1 site
C T G C A G G A C G T C
MluI  (4387)
1 site
A C G C G T T G C G C A
SexAI  (4354)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NruI  (833)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
EcoNI  (1170)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (1189)
1 site
C A A T T G G T T A A C
BbvCI  (1424)
1 site
C C T C A G C G G A G T C G
Bpu10I  (1424)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (1577)
1 site
C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (1934)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspDI  (2180)
1 site
A T C G A T T A G C T A
ClaI  (2180)
1 site
A T C G A T T A G C T A
NdeI  (2374)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
EcoRI  (2803)
1 site
G A A T T C C T T A A G
PaeR7I  (2809)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2809)
1 site
C T C G A G G A G C T C
SpeI  (2815)
1 site
A C T A G T T G A T C A
XbaI  (2827)
1 site
T C T A G A A G A T C T
NotI  (3156)
1 site
G C G G C C G C C G C C G G C G
BamHI  (3163)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PspOMI  (3294)
1 site
G G G C C C C C C G G G
ApaI  (3298)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (3332)
1 site
C C T A G G G G A T C C
BmgBI  (3724)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PflFI  (3829)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3829)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (3843)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3903)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3921)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
MscI  (4162)
1 site
T G G C C A A C C G G T
* Blocked by Dcm methylation.
AmpR
7116 .. 7976  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   7116 .. 7907  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistan