Resources
Plasmid Files

pLenti-C-Myc-DDK-IRES-GFP

Third-generation lentiviral PrecisionShuttle™ vector with a TurboGFP marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLenti-C-Myc-DDK-IRES-GFP.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
Download Free Trial Get SnapGene Viewer


BsmBI (7144) PasI (7137) ScaI (6955) DrdI (6309) Bsu36I (5625) BsrGI (4785) BsaBI * (4540) BlpI (4251) BstXI (4076) SgrDI (1) EcoNI (1550) BbvCI (1804) AleI (1957) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI - SgfI (3324) MreI - SgrAI (3326) AscI (3330) NheI (3355) BmtI (3359) RsrII (3361) MluI (3367) BsiWI (3370) PaeR7I - PspXI - XhoI (3382) PmeI (3460) BmgBI (4033) pLenti-C-Myc-DDK-IRES-GFP 7719 bp
BsmBI  (7144)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (7137)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
ScaI  (6955)
1 site
A G T A C T T C A T G A
DrdI  (6309)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (5625)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (4785)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BsaBI  (4540)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BlpI  (4251)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BstXI  (4076)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
AleI  (1957)
1 site
C A C N N N N G T G G T G N N N N C A C
XbaI  (2613)
1 site
T C T A G A A G A T C T
EcoRI  (3279)
1 site
G A A T T C C T T A A G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AsiSI  (3324)
1 site
G C G A T C G C C G C T A G C G
SgfI  (3324)
1 site
G C G A T C G C C G C T A G C G
MreI  (3326)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (3326)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (3330)
1 site
G G C G C G C C C C G C G C G G
NheI  (3355)
1 site
G C T A G C C G A T C G
BmtI  (3359)
1 site