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pLenti-C-Myc-DDK

Third-generation lentiviral vector for expression and dual tagging of an ORF. Can be used as an entry vector for the PrecisionShuttle™ system.

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pLenti-C-Myc-DDK.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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AclI (5977) BsmBI (5838) PasI (5831) MscI (5799) ScaI (5649) PspFI (5203) BseYI (5199) DrdI (5003) Bsu36I (4319) BsrGI (3479) PmeI (3460) PaeR7I - PspXI - XhoI (3382) NotI (3376) BsiWI (3370) MluI (3367) RsrII (3361) BmtI (3359) NheI (3355) AscI (3330) MreI - SgrAI (3326) AsiSI - SgfI (3324) BamHI (3292) SgrDI (1) KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) XmnI (1224) BspQI - SapI (1482) EcoNI (1550) BbvCI (1804) AleI (1957) PstI (2417) PspOMI (2605) ApaI (2609) XbaI (2613) EcoRI (3279) pLenti-C-Myc-DDK 6413 bp
AclI  (5977)
1 site
A A C G T T T T G C A A
BsmBI  (5838)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (5831)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MscI  (5799)
1 site
T G G C C A A C C G G T
ScaI  (5649)
1 site
A G T A C T T C A T G A
PspFI  (5203)
1 site
C C C A G C G G G T C G
BseYI  (5199)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
DrdI  (5003)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (4319)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (3479)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PmeI  (3460)
1 site
G T T T A A A C C A A A T T T G
PaeR7I  (3382)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3382)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3382)
1 site
C T C G A G G A G C T C
NotI  (3376)
1 site
G C G G C C G C C G C C G G C G
BsiWI  (3370)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
MluI  (3367)
1 site
A C G C G T T G C G C A
RsrII  (3361)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BmtI  (3359)
1 site
G C T A G C C G A T C G
NheI  (3355)
1 site
G C T A G C C G A T C G
AscI  (3330)
1 site
G G C G C G C C C C G C G C G G
MreI  (3326)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (3326)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AsiSI  (3324)
1 site
G C G A T C G C C G C T A G C G
SgfI  (3324)
1 site
G C G A T C G C C G C T A G C G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
KasI  (1017)
1 site
G G C G C C C C G C G G
NarI  (1018)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1019)
1 site
G G C G C C C C G C G G
PluTI  (1021)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XmnI  (1224)
1 site
G A A N N N N T T C C T T N N N N A A G
BspQI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
AleI  (1957)
1 site
C A C N N N N G T G G T G N N