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Plasmid Files

pLenti-C-Myc-DDK

Third-generation lentiviral vector for expression and dual tagging of an ORF. Can be used as an entry vector for the PrecisionShuttle™ system.

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pLenti-C-Myc-DDK Sequence and MappLenti-C-Myc-DDK.dna
Map and Sequence File   
Sequence Author:  OriGene
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 AclI (5977) BsmBI (5838) PasI (5831) MscI (5799) ScaI (5649) PspFI (5203) BseYI (5199) DrdI (5003) Bsu36I (4319) BsrGI (3479) PmeI (3460) PaeR7I - PspXI - XhoI (3382) NotI (3376) BsiWI (3370) MluI (3367) RsrII (3361) BmtI (3359) NheI (3355) AscI (3330) MreI - SgrAI (3326) AsiSI - SgfI (3324) BamHI (3292) EcoRI (3279) SgrDI (1) CMV enhancer KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) XmnI (1224) BspQI - SapI (1482) EcoNI (1550) BbvCI (1804) AleI (1957) PstI (2417) PspOMI (2605) ApaI (2609) XbaI (2613) CMV enhancer T7 promoter pLenti-C-Myc-DDK 6413 bp
AclI  (5977)
1 site
A A C G T T T T G C A A
BsmBI  (5838)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (5831)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MscI  (5799)
1 site
T G G C C A A C C G G T
ScaI  (5649)
1 site
A G T A C T T C A T G A
PspFI  (5203)
1 site
C C C A G C G G G T C G
BseYI  (5199)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
DrdI  (5003)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Bsu36I  (4319)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (3479)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PmeI  (3460)
1 site
G T T T A A A C C A A A T T T G
PaeR7I  (3382)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3382)
1 site
V C T C G A G B B G A G C T C V
XhoI  (3382)
1 site
C T C G A G G A G C T C
NotI  (3376)
1 site
G C G G C C G C C G C C G G C G
BsiWI  (3370)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
MluI  (3367)
1 site
A C G C G T T G C G C A
RsrII  (3361)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII
recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BmtI  (3359)
1 site
G C T A G C C G A T C G
NheI  (3355)
1 site
G C T A G C C G A T C G
AscI  (3330)
1 site
G G C G C G C C C C G C G C G G
MreI  (3326)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (3326)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AsiSI  (3324)
1 site
G C G A T C G C C G C T A G C G
SgfI  (3324)
1 site
G C G A T C G C C G C T A G C G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (3279)
1 site
G A A T T C C T T A A G
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
KasI  (1017)
1 site
G G C G C C C C G C G G
NarI  (1018)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
SfoI  (1019)
1 site
G G C G C C C C G C G G
PluTI  (1021)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
XmnI  (1224)
1 site
G A A N N N N T T C C T T N N N N A A G
BspQI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1482)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
AleI  (1957)
1 site
C A C N N N N G T G G T G N N N N C A C
PstI  (2417)
1 site
C T G C A G G A C G T C
PspOMI  (2605)
1 site
G G G C C C C C C G G G
ApaI  (2609)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
XbaI  (2613)
1 site
T C T A G A A G A T C T
CmR
5621 .. 6280  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
5621 .. 6280  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
WPRE
3597 .. 4185  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
3597 .. 4185  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
4956 .. 5544  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4956 .. 5544  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
237 .. 616  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2643 .. 3022  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2643 .. 3022  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1683 .. 1916  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
CMV promoter
3023 .. 3226  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
3023 .. 3226  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
618 .. 816  =  199 bp
human cytomegalovirus (CMV) immediate early
promoter
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
834 .. 1014  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
4714 .. 4894  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
3' LTR (truncated)
4714 .. 4894  =  181 bp
truncated 3' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
1061 .. 1186  =  126 bp
packaging signal of human immunodeficiency virus
type 1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
cPPT/CTS
2443 .. 2560  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
MCS
3279 .. 3387  =  109 bp
multiple cloning site
MCS
3279 .. 3387  =  109 bp
multiple cloning site
AmpR promoter
6281 .. 6385  =  105 bp
AmpR promoter
6281 .. 6385  =  105 bp
loxP
3508 .. 3541  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
3508 .. 3541  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
FLAG
3433 .. 3456  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
FLAG
3433 .. 3456  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
T7 promoter
3252 .. 3270  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
3252 .. 3270  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Myc
3385 .. 3414  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
Myc
3385 .. 3414  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc oncogene) epitope tag
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