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pLenti3.3/TR

Lentiviral vector for constitutive high-level expression of the Tet repressor in the T-REx™ system.

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pLenti3.3 TR.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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PciI (8818) AhdI (7930) DraIII (6790) SfiI (6302) RsrII (5527) PflFI - Tth111I (5129) MscI (5093) BsaBI * (4870) NotI (920) BbvCI (1196) HpaI (1800) BspDI - ClaI (1923) BsmBI (2472) SpeI (2534) BstXI (2547) EcoRV (2563) PspOMI (3094) ApaI (3098) AfeI (3627) MluI (3871) PflMI (4138) PfoI (4170) SacII (4400) SexAI * (4568) pLenti3.3/TR 9228 bp
PciI  (8818)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (7930)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6790)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (6302)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
RsrII  (5527)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (5129)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5129)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (5093)
1 site
T G G C C A A C C G G T
BsaBI  (4870)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
NotI  (920)
1 site