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pRetroQ-AcGFP1-C1

Self-inactivating retroviral vector for expressing a protein fused to the C-terminus of AcGFP1.

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pRetroQ-AcGFP1-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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SspI (7269) XmnI (7064) ScaI (6945) FspI (6687) AflIII - PciI (5572) BspQI - SapI (5456) AvrII (5325) SfiI (5278) EcoRV (4201) RsrII (3645) AscI (542) PshAI (676) NheI (2172) BmtI (2176) PflMI (2892) BglII (2920) PaeR7I - XhoI (2924) BstBI (2938) EcoRI (2940) SalI (2950) AccI (2951) PspOMI (2964) ApaI (2968) BamHI (2971) XbaI * (2983) BclI * (2993) BlpI (3282) BsmI (3440) BfuAI - BspMI (3518) BsiWI (3585) pRetroQ-AcGFP1-C1 7614 bp
SspI  (7269)
1 site
A A T A T T T T A T A A
XmnI  (7064)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (6945)
1 site
A G T A C T T C A T G A
FspI  (6687)
1 site
T G C G C A A C G C G T
AflIII  (5572)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (5572)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5456)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5456)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AvrII  (5325)
1 site
C C T A G G G G A T C C
SfiI  (5278)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (4201)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
RsrII  (3645)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AscI  (542)
1 site
G G C G C G C C C C G C G C G G
PshAI  (676)
1