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ptTS-Neo

Vector for expressing tTS, which represses expression from Tet-responsive promoters in the absence of doxycycline.

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ptTS-Neo.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoNI (4) XmnI (5977) PvuI (5748) BsaI (5439) AhdI (5378) Bpu10I (4182) HindIII (4159) PflMI (3912) BglII (3705) Bsu36I (3635) ApaI (3535) PspOMI (3531) PsiI (3364) MfeI (3356) NsiI (61) SfiI (192) StuI (238) BsaBI * (276) EagI (323) KasI (416) NarI (417) SfoI (418) PluTI (420) PflFI - Tth111I (535) RsrII (933) BstBI (1099) BsrGI (1407) SpeI (1463) SnaBI (1804) Eco53kI (2030) SacI (2032) BsmBI (2053) BbsI (2098) SacII (2119) XbaI (2138) PmlI (2266) NheI (2785) BmtI (2789) AvaI - BsoBI (2829) BmeT110I (2830) BamHI (2971) KflI (3014) ptTS-Neo 6365 bp
EcoNI  (4)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XmnI  (5977)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (5748)
1 site
C G A T C G G C T A G C
BsaI  (5439)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5378)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bpu10I  (4182)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
HindIII  (4159)
1 site
A A G C T T T T C G A A
PflMI  (3912)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BglII  (3705)
1 site
A G A T C T T C T A G A
Bsu36I  (3635)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
ApaI  (3535)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (3531)
1 site
G G G C C C C C C G G G
PsiI  (3364)
1 site
T T A T A A A A T A T T
MfeI  (3356)
1 site
C A A T T G G T T A A C
NsiI  (61)
1 site
A T G C A T T A C G T A
SfiI  (192)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (238)
1 site
A G G C C T T C C G G A
BsaBI  (276)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
EagI  (323)
1 site
C G G C C G G C C G G C
KasI  (416)
1 site
G G C G C C C C G C G G
NarI  (417)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (418)
1 site
G G C G C C C C G C G G
PluTI  (420)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
PflFI  (535)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (535)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
RsrII  (933)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstBI  (1099)
1 site
T T C G A A A A G C T T
BsrGI  (1407)
1 site
T G T A C A A C A T G