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pYC6/CT

Centromeric vector with a blasticidin resistance marker for galactose-inducible expression of C-terminally tagged proteins in S. cerevisiae.

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pYC6 CT.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NaeI (4359) MreI - NgoMIV - SgrAI (4357) SwaI (3876) PsiI (3870) BsaAI - PmlI (3852) BspDI - ClaI (3832) BmtI (3827) NheI (3823) HincII (3404) BtgI - NcoI - StyI (3338) MscI (3337) NruI * (3109) BsaBI * (3042) BlpI (2942) DraIII (2938) BmgBI (2911) SmaI (2906) TspMI - XmaI (2904) BglII (2898) AccI (2889) BsaHI (2573) ScaI (2516) BstAPI (323) SspI (497) HindIII (501) Acc65I (507) KpnI (511) Eco53kI (515) SacI (517) BamHI (519) EcoRI (550) PstI (559) EcoRV (562) NotI (577) PaeR7I - PspXI - XhoI (583) XbaI (589) PspOMI (595) ApaI (599) BstBI (602) PmeI (682) BsrGI (874) PciI (1143) BciVI (1346) AlwNI (1559) BspHI (1863) AhdI (2036) BpmI (2106) BglI (2156) pYC6/CT 4504 bp
NaeI  (4359)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
MreI  (4357)
1 site
C G C C G G C G G C G G C C G C
NgoMIV  (4357)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SgrAI  (4357)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SwaI  (3876)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PsiI  (3870)
1 site
T T A T A A A A T A T T
BsaAI  (3852)
1 site
Y A C G T R R T G C A Y
PmlI  (3852)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BspDI  (3832)
1 site
A T C G A T T A G C T A
ClaI  (3832)
1 site
A T C G A T T A G C T A
BmtI  (3827)
1 site
G C T A G C C G A T C G
NheI  (3823)
1 site
G C T A G C C G A T C G
HincII  (3404)
1 site
G T Y R A C C A R Y T G
BtgI  (3338)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (3338)
1 site
C C A T G G G G T A C C
StyI  (3338)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (3337)
1 site
T G G C C A A C C G G T
NruI  (3109)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
BsaBI  (3042)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BlpI  (2942)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
DraIII  (2938)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BmgBI  (2911)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SmaI  (2906)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2904)
1 site
C C C G G G G G G C C C
XmaI  (2904)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BglII  (2898)
1 site
A G A T C T T C T A G A
AccI  (2889)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (2573)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
ScaI  (2516)
1 site
A G T A C T T C A T G A
BstAPI  (323)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
SspI  (497)
1 site
A A T A T T T T A T A A
HindIII  (501)
1 site
A A G C T T T T C G A A
Acc65I  (507)
1 site
G G T A C C C C A T G G
KpnI  (511)
1 site
G G T A C C C C A T G G
Eco53kI  (515)
1 site
G A G C T C C T C G A G
SacI  (517)
1 site
G A G C T C C T C G A G