TA and GC Cloning

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System Requirements
OS Windows 7 or later
macOS 10.8 or later
Ubuntu Linux 14.04 or later
Fedora Linux 21 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

TA cloning is used to clone PCR products. It takes advantage of the 3' A overhangs added during amplification by Taq and some other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3' T overhangs. GC cloning is similar, and is based on the finding that Taq actually adds a mixture of 3' A and 3' G overhangs.

SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.

Please click below to see screenshots and a brief tutorial video. A list of annotated TA and GC cloning vectors is available online.


TA and GC Cloning Tutorial Video

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