TA and GC Cloning

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System Requirements
OS Windows 7 or later
macOS 10.10 or later
Fedora Linux 21 or later
Red Hat Linux 7.2 or later
Ubuntu Linux 14.04 or later
Memory1 GB RAM
Hard Disk250 MB available disk space
Display1024 x 768 or higher resolution

TA cloning is used to clone PCR products. It takes advantage of the 3' A overhangs added during amplification by Taq and some other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3' T overhangs. GC cloning is similar, and is based on the finding that Taq actually adds a mixture of 3' A and 3' G overhangs.

SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.

Please click below to see screenshots and a brief tutorial video. A list of annotated TA and GC cloning vectors is available online.


TA and GC Cloning Tutorial Video

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