StayGold

Bright and highly photostable dimeric green fluorescent protein from Cytaeis uchidae.
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HomePlasmidsFluorescent Protein Genes & PlasmidsStayGold
600 400 200 End (651) Esp3I - BsmBI (625) TsoI (550) BfuAI - BspMI (549) EarI (533) BlpI (475) BsgI (436) PmlI (430) MslI (394) BspHI (308) TatI (266) AvaI - BsoBI (235) BsrBI (125) HindIII (51) Start (0) StayGold StayGold 651 bp
End  (651)
0 sites
Esp3I  (625)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
BsmBI  (625)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
TsoI  (550)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BfuAI  (549)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (549)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
EarI  (533)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BlpI  (475)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BsgI  (436)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PmlI  (430)
1 site		 C A C G T G G T G C A C
MslI  (394)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
BspHI  (308)
1 site		 T C A T G A A G T A C T
TatI  (266)
1 site		 W G T A C W W C A T G W
AvaI  (235)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (235)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BsrBI  (125)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
HindIII  (51)
1 site		 A A G C T T T T C G A A
Start  (0)
0 sites
StayGold
1 .. 651  =  651 bp
217 amino acids  =  24.6 kDa
Product: bright and highly photostable dimeric green fluorescent protein (Hirano et al., 2022)
engineered variant of a GFP from Cytaeis uchidae
StayGold
1 .. 651  =  651 bp
217 amino acids  =  24.6 kDa
Product: bright and highly photostable dimeric green fluorescent protein (Hirano et al., 2022)
engineered variant of a GFP from Cytaeis uchidae
ORF:  1 .. 651  =  651 bp
ORF:  217 amino acids  =  24.6 kDa
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Download StayGold.dna file

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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.2
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos4a
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4b
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEYFP
pDsRed2-1
pPSmOrange-N1
CyOFP1
mgfp5
pDsRed2-C1
pRSET-BFP
CyPet
mGold
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGreenLantern
pE2-Crimson
pRSET-EmGFP
CyRFP1
mHoneydew
pE2-Crimson-C1
PS-CFP2
d1EGFP
MiCy
pE2-Crimson-N1
PSmOrange
d2ECFP
mIFP
pECFP
PSmOrange2
d2EGFP
miniSOG
pECFP-1
pTagBFP-C
d2EYFP
miRFP670
pECFP-C1
pTagBFP-N
d4EGFP
miRFP703
pECFP-N1
pTagCFP-C
daGFP
miRFP709
pEGFP
pTagCFP-N
Dendra2
mKalama1
pEGFP-1
pTagGFP2-C
dKeima-Red
mKate2
pEGFP-C1
pTagGFP2-N
dKeima570
mKeima-Red
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKikGR1
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKO
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO2
pEGFP-N2
pTagYFP-N
DsRed-Express2
mMaple
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaroon1
pEYFP
ptdTomato
DsRed-Monomer
mMiCy1
pEYFP-1
ptdTomato-C1
DsRed.T3
mNectarine
pEYFP-C1
pTimer
DsRed1
mNeonGreen
pEYFP-N1
pTimer-1
DsRed2
mNeptune
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune2
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2.5
pFusionRed-N
pTurboFP602-N
E2-Orange
mOrange
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange2
pGFPuv
pTurboGFP-B
EBFP
moxGFP
pGLO
pTurboGFP-C
EBFP2
mPapaya1
pHcRed1
pTurboGFP-N
ECFP
mPlum
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mRaspberry
pHcRed1-C1
pTurboRFP-B
EGFP
mRFP1
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRuby
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby2
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby3
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mScarlet
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet-2I
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-H
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-I
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet3
phmKO1-S1
pZsGreen1-1
Gamillus
mseCFP
phmUkG1-S1
pZsGreen1-C1
GFP
msGFP2
pHRed
pZsGreen1-N1
GFPuv
mStayGold (E138D)
pHTomato
pZsYellow
HcRed1
mStayGold(J)
pHuji
pZsYellow1-C1
hdKeima-Red
mTagBFP2
pKaede-S1
pZsYellow1-N1
hdKeima570
mTangerine
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTFP1
pKatushka2S-C
Rhacostoma GFP
hKO
mTurquoise
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise2
pKikGR1-S1
rsEGFP2
hMGFP
mUkG1
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mVenus
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mWasabi
pKillerOrange-N
rsFusionRed3
hmKikGR1
PA-GFP
pKillerRed-B
rsTagRFP
hmKO
PA-TagRFP
pKillerRed-C
SBFP2
hmKO2
pAcGFP1
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1-1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-C In-Fusion Ready
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C1
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C2
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C3
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-N In-Fusion Ready
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N1
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N2
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N3
pmCherry-1
SuperNova
iRFP713
pAG-S1
pmCherry-C1
SuperNova2
iRFP720
PAmCherry
pmCherry-N1
SYFP2
Kaede
PAmCherry1
pMFAPalpha2
TagBFP
Katushka2S
pAmCyan
pMFAPbeta1
TagCFP
KikGR1
pAmCyan1-C1
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-N1
pmKate2-C
TagRFP
KillerRed
PAmKate
pmKate2-N
TagRFP-T
Kohinoor
pAsRed2
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2-C1
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-N1
pmKO1-S1
TDsmURFP
LSSmKate1
pd1EGFP-N1
pmKO2-S1
tdTomato
LSSmKate2
pd2ECFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2EGFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EYFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd4EGFP-N1
pmOrange2-C1
TurboRFP
mApple
pDendra2
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2-C
pmPlum
UnaG
mBaoJin
pDendra2-N
pmRaspberry
Venus
mCardinal
pDG1-S1
pmStrawberry
Y-FAST
mCarmine
pDG3-S1
pmUkG1-S1
yeGFP
mCerulean
pdKeima-Red-S1
pNirFP-C
YFP
mCherry
pdKeima570-S1
pNirFP-N
YPet
mCherry2
pDsRed-Express
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express-1
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-C1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-N1
pPAmCherry-N1
ZsYellow1
mClover3

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