mScarlet3

Bright cysteine-free monomeric red fluorescent protein with fast and complete maturation.
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No matches
600 400 200 End (687) BsaWI (676) BsrFI - SgrAI (667) AleI (628) BsgI (580) MmeI (575) SfcI (540) EciI (538) NotI EagI (510) AatII (464) ZraI (462) BsrBI (444) PspFI (429) BseYI (425) BbsI (421) SacII (375) MspA1I (374) EcoO109I * (209) AhdI (180) StyI (146) BstEII (141) BbvCI - Bpu10I (136) BssSI - BssSαI (73) BanII (61) BsiHKAI (50) ApaLI (46) BtsI - BtsαI (23) BclI * (20) Start (0) mScarlet3 mScarlet3 687 bp
End  (687)
0 sites
BsaWI  (676)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BsrFI  (667)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (667)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AleI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
BsgI  (580)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (575)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SfcI  (540)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EciI  (538)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
NotI  (510)
1 site
G C G G C C G C C G C C G G C G
EagI  (510)
1 site
C G G C C G G C C G G C
AatII  (464)
1 site
G A C G T C C T G C A G
ZraI  (462)
1 site
G A C G T C C T G C A G
BsrBI  (444)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
PspFI  (429)
1 site
C C C A G C G G G T C G
BseYI  (425)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SacII  (375)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
MspA1I  (374)
1 site
C M G C K G G K C G M C
EcoO109I  (209)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (146)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BstEII  (141)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BbvCI  (136)
1 site
C C T C A G C G G A G T C G
Bpu10I  (136)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BssSI  (73)
1 site
C A C G A G G T G C T C
BssSαI  (73)
1 site
C A C G A G G T G C T C
BanII  (61)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (50)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (46)
1 site
G T G C A C C A C G T G
BtsI  (23)
1 site
G C A G T G N N C G T C A C
BtsαI  (23)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BclI  (20)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
mScarlet3
1 .. 687  =  687 bp
229 amino acids  =  25.9 kDa
Product: bright cysteine-free monomeric red fluorescent protein with fast and complete maturation (Gadella et al., 2023)
mScarlet3
1 .. 687  =  687 bp
229 amino acids  =  25.9 kDa
Product: bright cysteine-free monomeric red fluorescent protein with fast and complete maturation (Gadella et al., 2023)
ORF:  1 .. 687  =  687 bp
ORF:  229 amino acids  =  25.9 kDa
ORF:  1 .. 687  =  687 bp
ORF:  229 amino acids  =  22.8 kDa  (no start codon)
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Download mScarlet3.dna file

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