Simulate Gateway Cloning

Learn how SnapGene can simulate standard or MultiSite Gateway cloning.

Gateway Cloning

Plasmids can be constructed without restriction enzymes using Gateway® cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously.

Gateway Cloning

SnapGene simplifies Gateway cloning by automating the primer design. To plan a Gateway cloning procedure, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

A list of annotated Gateway cloning vectors is available online, and is embedded in the SnapGene software.

Gateway in SnapGene Full

Simulate Gateway Cloning in SnapGene

Learn how SnapGene can simulate standard or MultiSite Gateway cloning.

Gateway cloning does not require restriction enzymes to insert DNA into a plasmid. Instead, Gateway Cloning uses a two-step recombination process. This video talks you through the steps needed to simulate Gateway Cloning in SnapGene.

Simulate Gateway Cloning with Multiple Inserts

Learn how to simulate gateway cloning with multiple inserts in SnapGene.

Learn more

► Simulating Cloning in SnapGene

► SnapGene User Guide

► Gateway Cloning Guide

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