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Simulate Gateway Cloning

Learn how SnapGene can simulate standard or MultiSite Gateway cloning.

Gateway Cloning

Plasmids can be constructed without restriction enzymes using Gateway® cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously.

SnapGene simplifies Gateway cloning by automating the primer design. To plan a Gateway cloning procedure, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

A list of annotated Gateway cloning vectors is available online and is embedded in the SnapGene software.