SnapGene Updates

The latest SnapGene version is 7.1.1.

Update to SnapGene 7

Subscribers

Update for free by downloading the installer for your system (instructions):

Windows|Mac|RedHat|Ubuntu

Perpetual Customers

Contact us at  support.snapgene.com  to learn about upgrade options.

Release Notes

December 18, 2023

This release includes a number of important fixes and improves overall stability.

November 28, 2023

SnapGene 7.1 provides enhancements to streamline file search within a selected folder, introduces secondary structure visualizations for ssDNA sequences including primers, and includes a variety of fixes to improve stability, file import workflows and other functions.

File Search Enhancements

Quickly and easily build search queries to find your sequences and other files in the selected project folder with simplified filter entry controls, and run multiple searches in different tabs.

Quick Build Search Queries
Simplified Filter Controls and Multiple Tabs

Secondary Structure View for Single-Stranded DNA Sequences

Visualize the secondary structure for single-stranded DNA sequences calculated with the ViennaRNA package via the new secondary structure tab

Secondary Structure of ssDNA

View Primer Hairpins and Secondary Structures

Check your primers for hairpins or other self-binding secondary structures with a new button for calculating secondary structures for primer monomers, available in the primers tab.

Primers Secondary Structures

October 10, 2023

This release includes a number of stability and performance improvements as well as other fixes.

July 26, 2023

This release includes a number of important fixes and improves overall stability.

June 19, 2023

This release includes a number of important fixes and improves overall stability.

June 06, 2023

SnapGene 7.0 introduces an updated look and feel with enhanced data management capabilities. Easily navigate files and folders with tabs, and perform sequence and metadata-based searches for files directly from the new folder panel. Other enhancements include new primer insertion annotations and various usability improvements.

Tabbed Interface

Navigate between documents using the new tabbed interface, or pop out documents into separate windows for comparison purposes.

Tabbed Interface@2x

File and Folder Management

Choose any folder on your file system as a project and quickly view subfolders and files directly within a new, collapsible, folder panel. Perform standard file operations and quickly browse all supported file types.

File and Folder Management 2xn

File Search

Quickly find files within a project using attributes such as the name or type, containing sequence, feature annotations and more via a flexible, modern interface.

Search@2x
Search Results@1x

Primer Annotations

Visualize insertions such as enzymes, codon or short peptide sequences added to your primers in the updated Add/Edit Primer dialog. Regions of interest (Golden Gate restriction sites, Gateway cloning attB sites, directional TOPO motif) are now annotated for primers designed automatically as well.

Primer Annotations@2x

Preview Detected Features for New Sequences

The new file pane now includes a sequence map to preview features that are detected in your sequence and allows you to choose which annotations to include in your sequence.

Preview Detected Features@2x

Get Started

Quickly access recent files and folders using the new Get Started pane, or learn more about how to use SnapGene with links to short video tutorials, user guide articles, and the SnapGene Academy.

Getting Started@2x

Search for and Import Sequences from Dotmatics Bioregister

Quickly and easily search for sequences in Dotmatics Bioregister directly from within SnapGene based on the sequence name, Bioregister ID, or a similar sequence (BLAST search). Save a sequence of interest locally or open it in SnapGene right away. This integration requires Bioregister 6.1.0 or later.

Bioregister Search by Sequence@1x
Bioregister Search by ID@1x

January 09, 2023

Version 6.2 adds support for suboptimal RNA secondary structures and includes a number of other enhancements to the Structure view, includes a number of improvements to the Golden Gate cloning simulation tools and PCR simulations in general, provides better flexibility when working with Protein properties, and modernized buttons and icons.

RNA Secondary Structure view enhancements

Suboptimal RNA secondary structures are now shown. All structures can be recalculated using adjusted Tm and other settings. Visualization and selection capabilities have been enhanced, including the ability to display and make sequence selections that are synced across all views. Coordinates and 5' / 3' end labels can optionally be displayed. See View Predicted RNA Secondary Structure for more information.

Secondary Structure Enhancements@1x

Golden Gate Assembly Improvements

Various enhancements to the Golden Gate cloning dialogs include a ligation fidelity matrix for assessing overall reaction fidelity, it is now possible to adjust overhangs for manually designed primers, a new option to cut the vector with any enzymes (not just Type IIS enzymes), as well as an updated settings dialog with easy access to recommended enzymes.

Golden Gate Ligation Fidelity@1x

Improvements to primer design in PCR cloning simulations

You can now adjust the hybridization region for all PCR cloning simulations (e.g. PCR, Golden Gate, Gibson, In Fusion, etc.). When using automatic primer design the hybridized region will be configured automatically, ensuring miscellaneous features are not transferred to the product when a 5’ primer extension by chance partially hybridizes to the template.

Adjust Hybridized Region@1x

Protein properties can now be copied and exported

Copy or export the properties and amino acid data for full protein sequences or selected regions in text or spreadsheet (*.csv / *.tsv) formats. Individual properties can also be copied directly from the view.

Modernized toolbar, application, and document icons

SnapGene now uses modernized icons in the top toolbar. The application icon and file icons have also been updated.

Modernized Icons@1x

January 09, 2023

Version 6.2.1. enables registering sequences in Dotmatics Bioregister in addition to a fixing various other issues.

Register in Dotmatics Bioregister

Quickly and easily register your sequences in Dotmatics Bioregister directly from within SnapGene. It is no longer necessary to first save using a specific file format and manually upload to Bioregister using a web browser. This integration requires Bioregister 22.2 or later.

Domatics Bioregister@1x

December 05, 2022

Version 6.2 adds support for suboptimal RNA secondary structures and includes a number of other enhancements to the Structure view, includes a number of improvements to the Golden Gate cloning simulation tools and PCR simulations in general, provides better flexibility when working with Protein properties, and modernized buttons and icons.

RNA Secondary Structure view enhancements

Suboptimal RNA secondary structures are now shown. All structures can be recalculated using adjusted Tm and other settings. Visualization and selection capabilities have been enhanced, including the ability to display and make sequence selections that are synced across all views. Coordinates and 5' / 3' end labels can optionally be displayed. See View Predicted RNA Secondary Structure for more information.

Secondary Structure Enhancements

Golden Gate Assembly Improvements

Various enhancements to the Golden Gate cloning dialogs include a ligation fidelity matrix for assessing overall reaction fidelity, it is now possible to adjust overhangs for manually designed primers, a new option to cut the vector with any enzymes (not just Type IIS enzymes), as well as an updated settings dialog with easy access to recommended enzymes.

Golden Gate Ligation Fidelity@2x

Improvements to primer design in PCR cloning simulations

You can now adjust the hybridization region for all PCR cloning simulations (e.g. PCR, Golden Gate, Gibson, In Fusion, etc.). When using automatic primer design the hybridized region will be configured automatically, ensuring miscellaneous features are not transferred to the product when a 5’ primer extension by chance partially hybridizes to the template.

Adjust Hybridized Region

Protein properties can now be copied and exported

Copy or export the properties and amino acid data for full protein sequences or selected regions in text or spreadsheet (*.csv / *.tsv) formats. Individual properties can also be copied directly from the view.

Modernized toolbar, application, and document icons

SnapGene now uses modernized icons in the top toolbar. The application icon and file icons have also been updated.

Modernized Icons@2x

October 11, 2022

This release includes a number of important fixes.

August 16, 2022

This release includes stability improvements as well as fixes for cloning and viewing sequences.

July 04, 2022

Version 6.1 simplifies primer design when simulating Golden Gate Assembly, includes a new Secondary Structure view for ssRNA sequences, and supports Dark mode across all operating systems.

Golden Gate Assembly

Confidently simulate Golden Gate Assembly with a new tool which can automatically design primers and overhangs for Golden Gate, optimizing the reaction fidelity to maximize the likelihood of success.

Golden Gate Assembly

RNA Secondary Structure

Visualize how your single stranded RNA sequences will fold with a new Secondary Structure view which displays the optimal structure as calculated by the ViennaRNA.

RNA Secondary Structure

Dark Mode

The SnapGene user interface now supports dark mode and by default will match your Dark or Light mode operating system setting.

Dark Mode

Export Options

Customize how contents is exported to GenBank including LOCUS field identifier feature export options using the new Export pane in Preferences.

Export Options

Support for Apple Silicon

SnapGene now runs natively on Apple computers with M1 silicon chips.

July 03, 2022

This release includes an important fix for opening older files.

June 22, 2022

This release includes various important fixes.

May 12, 2022

This release includes important fixes including improved repairing of history in existing files.

May 02, 2022

This release includes a fix for a serious regression in version 6.0.3 that can result in history not being displayed correctly and ancestral sequences no longer being resurrectable.

April 17, 2022

This release includes a number of important stability and memory leak fixes and other optimizations.

January 12, 2022

This release includes an important stability fix.

January 11, 2022

This release significantly improves stability, improves performance, and fixes various issues.

November 28, 2021

Version 6.0.0 provides greater flexibility for working with cloning simulations, features, and agarose gels. Updates include new tools for silently adding or removing restriction sites, support for custom feature types, sharable agarose gel files, editable feature annotations in pairwise and multiple sequence alignments, and usability improvements including improved options for fragments chosen for cloning simulations.

Silent Mutation to Add or Remove Enzyme Sites

Restriction enzyme sites can now be automatically added or removed from a coding sequence by silent mutation.

Silent Mutation Remove Site

Support for Custom Feature Types

The set of available feature types can now be extended to include custom types (non-GenBank), and default feature colors can be changed.

Custom Feature Types

Agarose Gel Files

Agarose gel simulations can now be saved as .gel files, allowing gels to be viewed and edited later or shared with others.

Agarose Gel Files

Feature Annotation in Pairwise and Multiple Alignments

Editable features are now fully supported in both pairwise and multiple sequence alignments, with the ability to add, edit, and search from either the Alignment view or the new Features view.

Features in Alignments

Cloning Simulation Enhancements

New flexibility in cloning simulation dialogs enables fragments to be added, removed, or re-ordered within the cloning interface. Vectors can now be flipped when simulating restriction cloning, Gibson Assembly, In-Fusion Cloning, and NEBuilder HiFi Assembly.

Reorder Fragments