Simulate Golden Gate Assembly
Learn how SnapGene can simulate Golden Gate Assembly to assemble DNA fragments into a plasmid without using restriction enzymes.
Golden Gate Assembly
Golden Gate Assembly is a one-pot, one-step cloning procedure that uses Type IIS restriction enzymes to efficiently clone multiple inserts into a vector simultaneously.
SnapGene simplifies Golden Gate Assembly by automating the primer design. To plan a Golden Gate procedure, just select the DNA fragments that you wish to assemble, and SnapGene will design the primers and overhangs and optimize the reaction fidelity to maximize the likelihood of success.