Simulate Golden Gate Assembly

Learn how SnapGene can simulate Golden Gate Assembly to assemble DNA fragments into a plasmid without using restriction enzymes.

Golden Gate Assembly

Golden Gate Assembly is a one-pot, one-step cloning procedure that uses Type IIS restriction enzymes to efficiently clone multiple inserts into a vector simultaneously.

Golden Gate Assembly in SnapGeneGolden Gate Assembly

SnapGene simplifies Golden Gate Assembly by automating the primer design. To plan a Golden Gate procedure, just select the DNA fragments that you wish to assemble, and SnapGene will design the primers and overhangs and optimize the reaction fidelity to maximize the likelihood of success.

Golden Gate Assembly SnapGene interfaceGolden Gate Assembly in SnapGene

How to Simulate Golden Gate Assembly

How to simulate Golden Gate Assembly in SnapGene

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 Simulating Cloning in SnapGene

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► Golden Gate Assembly Guide

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