Simulate In-Fusion Cloning
Learn how SnapGene can simulate Takara’s In-Fusion cloning to insert one or more DNA fragments into a plasmid.
In-Fusion Cloning
Takara’s In-Fusion® cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene was the first software to simulate this procedure.
For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends.
SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers.
How to Simulate In-Fusion Cloning in SnapGene
Learn how SnapGene can simulate In-Fusion cloning to insert one or more DNA fragments into a plasmid.
This video will show you how to simulate Takara's In-Fusion Cloning with a single fragment in SnapGene. In-Fusion Cloning is a ligation-independent cloning technique that uses complementary annealing of an insert and a vector.
In-Fusion Cloning with Multiple Inserts
Learn how to simulate in-fusion cloning with multiple inserts by following this step-by-step tutorial.
Takara Recommends SnapGene
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► Simulating Cloning in SnapGene
► SnapGene User Guides
► In-Fusion Cloning Guide
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