Simulate In-Fusion Cloning
Learn how SnapGene can simulate Takara’s In-Fusion cloning to insert one or more DNA fragments into a plasmid.
In-Fusion Cloning
Takara’s In-Fusion® cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene was the first software to simulate this procedure.
For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends.
SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers.
How to Simulate In-Fusion Cloning in SnapGene
Learn how SnapGene can simulate In-Fusion cloning to insert one or more DNA fragments into a plasmid.
In-Fusion Cloning with Multiple Inserts
Takara Recommends SnapGene
Learn More
► Simulating Cloning in SnapGene
A video series on how to simulate a range of cloning techniques in SnapGene
► SnapGene User Guides
Step by step instructions for In-Fusion® Cloning with SnapGene
► In-Fusion Cloning Guide
An in-depth guide to performing In-Fusion® Cloning