Simulate TOPO Cloning

Learn how to simulate TOPO Cloning in SnapGene

TOPO Cloning

PCR products can be cloned at high efficiency using TOPO® cloning, which is performed with linearized vectors that have vaccinia virus topoisomerase I covalently bound to the 3’ phosphates. SnapGene simulates three different variations of this method.

  • TOPO TA cloning employs linearized vectors such as pcDNA™3.4 TOPO to clone PCR products with 3’-A overhangs. These PCR products are typically generated with Taq polymerase.
  • Blunt TOPO cloning employs linearized vectors such as pCR®-Blunt II-TOPO to clone PCR products with blunt overhangs. These PCR products are typically generated with high-fidelity polymerases.
  • Directional TOPO cloning employs linearized vectors such as pET200/D-TOPO to clone PCR products in a directional manner.

SnapGene simplifies TOPO cloning by automating the primer design. To plan a TOPO cloning procedure, select the DNA fragment that you wish to amplify, and choose a TOPO cloning vector from a list that is embedded in the software. SnapGene will then choose suitable primers.

Directional topoDirectional TOPO® cloning

Simulate TOPO Cloning in SnapGene

Learn how to simulate TOPO cloning in SnapGene with this step-by-step tutorial.

Learn More

► Simulate TA and GC Cloning

► Simulating Cloning in SnapGene

► SnapGene User Guides

► TOPO Cloning guide

► Polymerase Chain Reaction Guide

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