Simulate TOPO Cloning
Learn how to simulate TOPO Cloning in SnapGene
PCR products can be cloned at high efficiency using TOPO® cloning, which is performed with linearized vectors that have vaccinia virus topoisomerase I covalently bound to the 3’ phosphates. SnapGene simulates three different variations of this method.
- TOPO TA cloning employs linearized vectors such as pcDNA™3.4 TOPO to clone PCR products with 3’-A overhangs. These PCR products are typically generated with Taq polymerase.
- Blunt TOPO cloning employs linearized vectors such as pCR®-Blunt II-TOPO to clone PCR products with blunt overhangs. These PCR products are typically generated with high-fidelity polymerases.
- Directional TOPO cloning employs linearized vectors such as pET200/D-TOPO to clone PCR products in a directional manner.
SnapGene simplifies TOPO cloning by automating the primer design. To plan a TOPO cloning procedure, select the DNA fragment that you wish to amplify, and choose a TOPO cloning vector from a list that is embedded in the software. SnapGene will then choose suitable primers.
Simulate TOPO Cloning in SnapGene
Learn how to simulate TA and GC cloning in SnapGene
A video series on how to simulate a range of cloning techniques in SnapGene
Step by step instructions for TOPO cloning with SnapGene
An in-depth guide to performing TOPO cloning
An in-depth guide to the polymerase chain reaction (PCR)