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Gateway® Cloning

Plasmids can be constructed without restriction enzymes using Gateway cloning, which inserts DNA fragments by recombination. SnapGene simulates different variations of this method.

For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an Entry Vector. Then the fragment is transferred to a Destination Vector in an LR cloning reaction, creating the final Expression Vector. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously.

SnapGene simplifies Gateway cloning by automating the primer design. To plan a Gateway cloning procedure, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.

Please click below to see a screenshot and a brief tutorial video. A list of annotated Gateway cloning vectors is available online, and is embedded in the SnapGene software.

Gateway Cloning Tutorial Video