Many researchers are turning to Gibson Assembly to insert fragments into a plasmid without the use of restriction enzymes. SnapGene simulates this method with an intuitive interface.
For Gibson Assembly, the DNA segments to be joined are amplified by PCR to create overlapping ends. Then the amplified products are incubated with assembly enzymes, and the mixture is transformed into bacteria.
SnapGene simplifies Gibson Assembly by automating the primer design. To plan a Gibson Assembly reaction, just select the DNA fragments that you wish to join, and SnapGene will choose suitable primers.
Please click below to see a screenshot and a brief tutorial video.
Gibson Assembly Tutorial Video