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In-Fusion® Cloning

Clontech’s In-Fusion cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene was the first software to simulate this procedure.

For the In-Fusion reaction, a linearized vector is mixed with one or more PCR products that have overlapping ends.

SnapGene simplifies In-Fusion cloning by automating the primer design. To plan an In-Fusion reaction, just select the DNA fragments that you wish to fuse, and SnapGene will choose suitable primers.

Please click below to see a screenshot, a brief tutorial video, and In-Fusion-ready vectors from Clontech.

In-Fusion Cloning Tutorial Video