Learn Gateway Cloning
This in-depth course examines the Gateway Cloning technique. It includes a detailed overview, pros and cons, top tips and a how-to guide for using Gateway Cloning in SnapGene.
Course Content
Introduction to Gateway Cloning
The Gateway cloning method, developed by Invitrogen in the 1990s, uses the site-specific recombination machinery of phage lambda to integrate its genome into E. Coli.
LENGTH 4 Minutes
A Detailed Look at Gateway Cloning
Take a detailed look at ThermoFisher's proprietary system Gateway cloning. Learn how the 2-step BP and LR reactions work to make Gateway cloning an effective cloning technique.
LENGTH 6 Minutes
Gateway Cloning Pros and Cons
In this video, we discuss the relative pros and cons of Gateway Cloning. Pros include its speed, high throughput and the ability to combine multiple fragments in one reaction.
LENGTH 4 Minutes
Tips for Gateway Cloning
Now that you're familiar with the technique of Gateway Cloning take your learning further with these 5 tips for carrying out successful cloning experiments in the lab.
LENGTH 5 Minutes
Learn More About SnapGene
VIDEO SERIESGateway Cloning
Learn all the steps needed to simulate single or multisite Gateway Cloning in SnapGene.
GUIDEIntroduction to SnapGene
If you're new to SnapGene this brief guide will help you get started.
VIDEO SERIESGetting Started with SnapGene
Six short videos covering the core SnapGene workflows: interface navigation, file management, sequence annotation, and using the Actions and Tools menus to plan and verify cloning experiments.
GUIDEGateway Cloning Technique
An overview of Thermo Fisher's Gateway Cloning technique and how to simulate the Gateway reaction in SnapGene.
Discover the most user-friendly molecular biology experience.