The Industry’s Most Popular Molecular Cloning Tool

The easiest way to plan, visualize and document your everyday molecular cloning procedures

Improve accuracy while moving faster, so you save time and money.

  • Elegant, information-rich windows for simulating common cloning and PCR methods
  • Clear visual schematics let you see exactly how your construct will be put together
  • SnapGene helps you identify and avoid common mis-steps by keeping track of details like DNA methylation and phosphorylation

With unparalleled visibility and control, even novice cloners will quickly accomplish complex tasks.

  • Gain unparalleled visibility of your DNA or protein sequence
  • Create and browse richly annotated plasmid maps or scan large DNA sequences with thousands of annotated features
  • Confirm your cloning results with highly visual alignment of sequencing results to your simulated construct

Automatic documentation of simulated procedures saves time and makes sharing and saving work easier, more efficient, and more effective.

  • Automatically generate a rich graphical history of every sequence edit and cloning procedure
  • Enhance collaboration and retain knowledge by sharing constructs complete with the historical details of how it was made
  • Experiment confidently with comprehensive “undo” capabilities that retrace your steps
I just want to say that I LOVE SnapGene. It has dramatically altered the research that my lab does, giving us the confidence to take on difficult cloning with minimal effort!
D. Bryant
University of Glasgow

Beyond the Basics of Molecular Cloning

SnapGene is the most popular cloning tool for a reason. It’s fast, smart and extremely user-friendly

  • Intuitive technology identifies design flaws in cloning procedures so they can be corrected
  • Simulate standard PCR using your own primers, or allow SnapGene to design them automatically
  • Specialised cloning tools ensure fast accurate construct design for all major molecular cloning techniques
SnapGene is the most intuitive, easy to use cloning program I have ever been able to get my hands on.
D. Kraut
Villanova University

Verify your project design and prevent errors

Automatically View Plasmid Features

  • Annotate features on your plasmids using SnapGene’s curated feature database or your own custom features
  • Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view
  • Customize your maps with flexible annotation and visualization controls

Check Your Sequences with Alignment Tools

  • Validate your sequenced constructs match your simulated construct using the powerful Align to Reference tool
  • Align sequences using trusted algorithms for pairwise and multiple alignments, including Clustal Omega, MAFFT, MUSCLE and T-Coffee
  • Assemble Sanger sequencing reads into complete contigs using CAP3

Realistic Agarose Gel Simulations

  • Visualise exactly what you will see in the lab with SnapGene’s empirically based gel simulation algorithm
  • Flexible configuration of all gel elements, including number of lanes, % agarose, running time and a full set of MW markers
  • Record and identify your band of interest with detailed fragment information for each lane
Beautiful graphic interface especially for marking features and multiple sequence alignments. THANK YOU!!
S.R. Veazey
UT Health Science Center, San Antonio

Configured for automatic documentation and easy data exchange

Automatically Create a Graphical History

  • Record experimental procedures and changes to documents visually
  • Show history colors in history, map and sequence views
  • Fully resurrectable sequence history embedded in the document

Convert and Share Data

  • Store, search, share and organize your sequences, files and maps
  • Work collaboratively with sequences in collections stored on sharable drives, servers or cloud services
  • Easily read and export sequences, annotations and other metadata from many common file formats
The history feature (my favorite) keeps track of each step, and any construct I get from colleagues comes complete with how it was made. I recommend this software to anyone who clones DNA.
D. Strongin
Fred Hutchinson Cancer Center

All Features

Explore a list of SnapGene’s robust set of features designed to enhance your everyday
molecular cloning procedures

Molecular Cloning

  • Restriction Cloning
  • Gateway Cloning
  • Gibson Assembly
  • NEBuilder HiFi Assembly
  • In-Fusion Cloning
  • TA & GC Cloning
  • TOPO Cloning

Primers

  • Design primers
  • Anneal two oligos to form a double-stranded product

PCR and Mutagenesis

  • Simulate PCR
  • Overlap extension PCR
  • Primer-directed mutagenesis

Enzyme Sets

  • Predefined enzyme sets - by company or cutter
  • Create custom enzyme sets
  • View detailed enzyme information
  • Rich support for methylation sensitivity and associated error prevention

Convert File Formats

  • Alignment Formats
  • ApE
  • CLC Bio
  • Clone Manager
  • DNA Strider
  • DNADynamo
  • DNASIS
  • DNAssist
  • DNASTAR Lasergene®
  • DS Gene
  • EMBL (ENA)
  • EnzymeX
  • GenBank / DDBJ
  • Gene Construction Kit®
  • Geneious
  • GeneTool
  • Genome Compiler
  • Jellyfish
  • MacVector
  • pDRAW32
  • Serial Cloner
  • Swiss-Prot
  • Vector NTI®
  • Visual Cloning

Agarose Gel Simulation

  • Simulate an agarose gel
  • Simulate a Restriction Digest
  • Simulate a PCR Amplification
  • Large collection of MW markers

Features / Annotations

  • Create and edit features
  • Automatic annotation of common features
  • Annotate novel features manually
  • Choose Alternative Codons
  • Sophisticated numbering of feature translations
  • Support for ribosomal slippage

Translations

  • View and edit translated features
  • Open reading frames (ORFs)
  • Whole-sequence translations
  • Check reading frames for gene fusions
  • Make Protein (from DNA)
  • Reverse Translate (from Protein)

Alignment

  • Align DNA sequences with a reference sequence
  • Verify cloning or mutagenesis
  • Align cDNA to a chromosome
  • Pairwise and multi sequence DNA and Protein alignment
  • Choice of alignment algorithms - Clustal Omega, MAFFT, MUSCLE, T-Coffee
  • Contig Assembly

Visualizing

  • See multiple views of a DNA sequence
  • Large sequence support - browse chromosome size sequences
  • Edit DNA and protein sequences
  • Color code sequences

History Tracking

  • Comprehensive “undo” capability
  • See a graphical history of a product
  • Use optional history colors to identify the most recent change to a sequence

Data Management

  • Import from common file formats including annotations and notes
  • Export to standard formats
  • Create and share Collections
  • Share data with SnapGene Viewer
  • Run batch operations

Search

  • Search for DNA or protein sequences
  • Search for enzymes, features or primers

General

  • Cross platform compatibility - Windows, macOS, Linux