With SnapGene, ensure that a construct is what you want and confirm that you obtained the desired sequence.

Reading Frames for Gene Fusions

Ensure that the translated features in your construct are in frame.

Linked Translations
Linked Translations · Warning Messages

Use Sequence view to see at a glance whether two translated features are in frame. If so, the translations are linked on the same line. If not, the translations are on separate lines.

Alignment to a Reference Sequence

Use the powerful alignment tool to check whether an actual construct matches the simulated construct.

Map Overview
Map Overview · Sequence Details

See an overview of where the aligned sequences match the reference sequence, and where discrepancies exist.