Simulate TA and GC Cloning
Learn how SnapGene can simulate TA cloning or Lucigen’s GC cloning.
TA and GC Cloning
TA cloning is used to clone PCR products, taking advantage of the 3′ A overhangs added during amplification by Taq and other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3′ T overhangs. GC cloning is similar, and is based on the finding that Taq adds a mixture of 3′ A and 3′ G overhangs.
SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.
A list of annotated TA and GC cloning vectors is available online.
Simulate TA and GC Cloning in SnapGene
Learn how SnapGene can simulate TA cloning or Lucigen’s GC cloning.
Learn More
► Simulating Cloning in SnapGene
A video series on how to simulate a range of cloning techniques in SnapGene
► SnapGene User Guides
Step by step instructions for TA and GC cloning with SnapGene
► PCR Cloning Guide
An in-depth guide to the cloning with PCR
► Polymerase Chain Reaction Guide
An in-depth guide to the polymerase chain reaction (PCR)