Simulate TA and GC Cloning

Learn how SnapGene can simulate TA cloning or Lucigen’s GC cloning.

TA and GC Cloning

TA cloning is used to clone PCR products, taking advantage of the 3′ A overhangs added during amplification by Taq and other polymerases. These sticky ends enable insertion into a linearized vector with complementary 3′ T overhangs. GC cloning is similar, and is based on the finding that Taq adds a mixture of 3′ A and 3′ G overhangs.

TA Cloning

SnapGene provides a simple interface for simulating TA and GC cloning. A variety of commercially available linearized TA and GC cloning vectors are embedded in the software.

A list of annotated TA and GC cloning vectors is available online.

TA and GC in SnapGene full interface

Simulate TA and GC Cloning in SnapGene

Learn how SnapGene can simulate TA cloning or Lucigen’s GC cloning.

TA and GC cloning are two simple techniques that do not require restriction enzymes. This video will show you how to simulate these cloning techniques in SnapGene.

Learn More

► Simulating Cloning in SnapGene

► SnapGene User Guides

► PCR Cloning Guide

► Polymerase Chain Reaction Guide

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