pBK-CMV

Dual eukaryotic and prokaryotic expression vector.

Sequence Author: Agilent Technologies

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Explore Over 2.7k Plasmids: I.M.A.G.E. Consortium Plasmids | More Plasmid Sets
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HomePlasmidsI.M.A.G.E. Consortium PlasmidspBK-CMV
SfiI (4099) BseRI (4055) StuI (4050) PluTI (3872) SfoI (3870) NarI (3869) KasI (3868) MscI (3789) PflFI - Tth111I (3752) BsrDI (3640) RsrII (3352) BstBI (3187) PfoI (3091) BsaI (2878) ApaLI (2263) DraIII (240) MluI (463) BtsI - BtsαI (516) HpaI (590) MfeI (599) BclI * (692) PvuI (852) Acc65I (1015) KpnI (1019) TspMI - XmaI (1024) AleI - SmaI (1026) BstXI (1028) PspOMI (1042) ApaI (1046) NotI - SacII (1049) XbaI (1056) ScaI (1064) PaeR7I - XhoI (1068) HindIII (1074) EcoRI (1083) BamHI (1092) SpeI (1098) SalI (1104) AccI (1105) PstI - SbfI (1114) BssHII (1116) Eco53kI (1124) SacI (1126) NheI (1300) BmtI (1304) SnaBI (1555) NdeI (1659) AseI (1886) PciI (1949) pBK-CMV 4518 bp
SfiI  (4099)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (4055)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (4050)
1 site		 A G G C C T T C C G G A
PluTI  (3872)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3870)
1 site		 G G C G C C C C G C G G
NarI  (3869)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3868)
1 site		 G G C G C C C C G C G G
MscI  (3789)
1 site		 T G G C C A A C C G G T
PflFI  (3752)
1 site		 G A C N N N G T C C T G N N N C A G