pBK-CMV

Dual eukaryotic and prokaryotic expression vector.

Sequence Author: Agilent Technologies

|Download SnapGene Viewer
No matches
SfiI (4099) BseRI (4055) StuI (4050) PluTI (3872) SfoI (3870) NarI (3869) KasI (3868) MscI (3789) PflFI - Tth111I (3752) BsrDI (3640) RsrII (3352) BstBI (3187) PfoI (3091) BsaI (2878) ApaLI (2263) DraIII (240) MluI (463) BtsI - BtsαI (516) HpaI (590) MfeI (599) BclI * (692) PvuI (852) Acc65I (1015) KpnI (1019) TspMI - XmaI (1024) AleI - SmaI (1026) BstXI (1028) PspOMI (1042) ApaI (1046) NotI - SacII (1049) XbaI (1056) ScaI (1064) PaeR7I - XhoI (1068) HindIII (1074) EcoRI (1083) BamHI (1092) SpeI (1098) SalI (1104) AccI (1105) PstI - SbfI (1114) BssHII (1116) Eco53kI (1124) SacI (1126) NheI (1300) BmtI (1304) SnaBI (1555) NdeI (1659) AseI (1886) PciI (1949) pBK-CMV 4518 bp
SfiI  (4099)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI  (4055)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (4050)
1 site
A G G C C T T C C G G A
PluTI  (3872)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3870)
1 site
G G C G C C C C G C G G
NarI  (3869)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3868)
1 site
G G C G C C C C G C G G
MscI  (3789)
1 site
T G G C C A A C C G G T
PflFI  (3752)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3752)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrDI  (3640)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
RsrII  (3352)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstBI  (3187)
1 site
T T C G A A A A G C T T
PfoI  (3091)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BsaI  (2878)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ApaLI  (2263)
1 site
G T G C A C C A C G T G
DraIII  (240)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
MluI  (463)
1 site
A C G C G T T G C G C A
BtsI  (516)
1 site
G C A G T G N N C G T C A C
BtsαI  (516)
1 site
G C A G T G N