pCMV SPORT4

Vector for cloning and transient mammalian cell expression of cDNAs.

Sequence Author: I.M.A.G.E. Consortium

|Download SnapGene Viewer
No matches
BspDI * - ClaI * (123) NaeI (4258) NgoMIV (4256) DraIII (4155) PflMI * (3843) XmnI (3455) ScaI (3336) BsaI (2917) AhdI (2856) AlwNI (2337) PspFI (2229) BseYI (2225) PciI (1921) HpaI (259) MfeI (268) BsaBI * (360) BclI * (361) NheI (637) BmtI (641) T7 promoter SphI (731) MluI (733) HindIII (739) PspOMI (745) ApaI (749) AbsI - PaeR7I - PspXI - XhoI (751) BamHI (757) XbaI (763) EagI - NotI (770) SpeI (779) SalI (791) AccI (792) TspMI - XmaI (796) SmaI (798) EcoRI (801) RsrII (808) AgeI (810) Acc65I (813) KpnI (817) PstI - SbfI (822) BfuAI - BspMI (825) SP6 promoter StuI (865) M13 rev SacII (912) AvrII (915) BbsI (944) BsmBI - Esp3I (989) NcoI (1218) SnaBI (1242) NsiI (1529) BstAPI (1745) BspQI - SapI (1805) pCMV SPORT4 4387 bp
BspDI  (123)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (123)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NaeI  (4258)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4256)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (4155)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PflMI  (3843)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
XmnI  (3455)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3336)
1 site
A G T A C T T C A T G A
BsaI  (2917)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2856)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2337)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (2229)
1 site
C C C A G C G G G T C G
BseYI  (2225)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (1921)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
HpaI  (259)
1 site
G T T A A C C A A T T G
MfeI  (268)
1 site
C A A T T G G T T A A C
BsaBI  (360)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BclI  (361)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NheI  (637)
1 site
G C T A G C C G A T C G
BmtI  (641)
1 site
G C T A G C C G A T C G
SphI  (731)
1 site
G C A T G C C G T A C G
MluI  (733)
1 site
A C G C G T T G C G C A
HindIII  (739)
1 site
A A G C T T T T C G A A
PspOMI  (745)
1 site
G G G C C C C C C G G G
ApaI  (749)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AbsI  (751)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (751)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (751)
1 site
V C T C G A G B B G A G C T C V
XhoI  (751)
1 site
C T C G A G G A G C T C
BamHI  (757)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (763)
1 site
T C T A G A A G A T C T
EagI  (770)
1 site
C G G C C G G C C G G C
NotI  (770)
1 site
G C G G C C G C C G C C G G C G
SpeI  (779)
1 site
A C T A G T T G A T C A
SalI  (791)
1 site
G T C G A C C A G C T G
AccI  (792)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
TspMI  (796)
1 site
C C C G G G G G G C C C
XmaI  (796)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (798)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (801)
1 site
G A A T T C C T T A A G
RsrII  (808)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (810)
1 site
A C C G G T T G G C C A
Acc65I  (813)
1 site
G G T A C C C C A T G G
KpnI  (817)
1 site
G G T A C C C C A T G G
PstI  (822)
1 site
C T G C A G G A C G T C
SbfI  (822)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (825)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (825)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
StuI  (865)
1 site
A G G C C T T C C G G A
SacII  (912)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AvrII  (915)
1 site
C C T A G G G G A T C C
BbsI  (944)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsmBI  (989)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (989)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
NcoI  (1218)
1 site
C C A T G G G G T A C C
SnaBI  (1242)
1 site
T A C G T A A T G C A T
NsiI  (1529)
1 site
A T G C A T T A C G T A
BstAPI  (1745)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BspQI  (1805)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (1805)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AmpR
2783 .. 3643  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2783 .. 3574  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2783 .. 3643  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3575 .. 3643  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2783 .. 3643  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1982 .. 2570  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1982 .. 2570  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3931 .. 4386  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3931 .. 4386  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
1219 .. 1522  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1219 .. 1522  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
1015 .. 1218  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
1015 .. 1218  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
MCS
727 .. 824  =  98 bp
multiple cloning site
MCS
727 .. 824  =  98 bp
multiple cloning site
loxP
2648 .. 2681  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
2648 .. 2681  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
T7 promoter
691 .. 709  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
691 .. 709  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
832 .. 850  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
832 .. 850  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
873 .. 889  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
873 .. 889  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  3629 .. 3868  =  240 bp
ORF:  79 amino acids  =  9.0 kDa
ORF:  870 .. 1136  =  267 bp
ORF:  88 amino acids  =  9.5 kDa
ORF:  2913 .. 3179  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1325 .. 1582  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  2783 .. 3643  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pCMV SPORT4.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.