pSPORT 1

Vector for cloning, sequencing, and bacterial expression of cDNAs.

Sequence Author: Thermo Fisher (Invitrogen)

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M13 fwd NaeI (3980) NgoMIV (3978) DraIII (3877) PsiI (3749) XmnI (3333) ScaI (3214) TatI (3212) BsaI (2795) AhdI (2734) AlwNI (2257) SP6 promoter ZraI (193) AatII (195) SphI (201) MluI (203) BsiWI (206) SnaBI (210) HindIII (213) BamHI (219) XbaI (225) EagI - NotI (232) SpeI (241) Eco53kI (249) SacI (251) SalI (253) AccI (254) AvaI - BsoBI - TspMI - XmaI (258) SmaI (260) EcoRI (263) BspEI (267) RsrII (270) AgeI (272) Acc65I (275) KpnI (279) PstI - SbfI (284) BfuAI - BspMI (287) T7 promoter lac operator KasI (552) NarI * (553) SfoI (554) PluTI (556) BsmBI - Esp3I (576) EcoRV (745) PspOMI (984) ApaI (988) BstEII (1009) BclI * (1177) PflMI (1616) MauBI (1648) PciI (1841) pSPORT 1 4109 bp
NaeI  (3980)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3978)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (3877)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (3749)
1 site
T T A T A A A A T A T T
XmnI  (3333)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3214)
1 site
A G T A C T T C A T G A
TatI  (3212)
1 site
W G T A C W W C A T G W
BsaI  (2795)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2734)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2257)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ZraI  (193)
1 site
G A C G T C C T G C A G
AatII  (195)
1 site
G A C G T C C T G C A G
SphI  (201)
1 site
G C A T G C C G T A C G
MluI  (203)
1 site
A C G C G T T G C G C A
BsiWI  (206)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (210)
1 site
T A C G T A A T G C A T
HindIII  (213)
1 site
A A G C T T T T C G A A
BamHI  (219)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (225)
1 site
T C T A G A A G A T C T
EagI  (232)
1 site
C G G C C G G C C G G C
NotI  (232)
1 site
G C G G C C G C C G C C G G C G
SpeI  (241)
1 site
A C T A G T T G A T C A
Eco53kI  (249)
1 site
G A G C T C C T C G A G
SacI  (251)
1 site
G A G C T C C T C G A G
SalI  (253)
1 site
G T C G A C C A G C T G
AccI  (254)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AvaI  (258)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (258)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (258)
1 site
C C C G G G G G G C C C
XmaI  (258)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (260)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (263)
1 site
G A A T T C C T T A A G
BspEI  (267)
1 site
T C C G G A A G G C C T
RsrII  (270)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (272)
1 site
A C C G G T T G G C C A
Acc65I  (275)
1 site
G G T A C C C C A T G G
KpnI  (279)
1 site
G G T A C C C C A T G G
PstI  (284)
1 site
C T G C A G G A C G T C
SbfI  (284)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (287)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (287)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
KasI  (552)
1 site
G G C G C C C C G C G G
NarI  (553)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (554)
1 site
G G C G C C C C G C G G
PluTI  (556)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BsmBI  (576)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (576)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
EcoRV  (745)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PspOMI  (984)
1 site
G G G C C C C C C G G G
ApaI  (988)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BstEII  (1009)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BclI  (1177)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PflMI  (1616)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
MauBI  (1648)
1 site
C G C G C G C G G C G C G C G C
PciI  (1841)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
lacI
464 .. 1546  =  1083 bp
360 amino acids  =  38.5 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
464 .. 1546  =  1083 bp
360 amino acids  =  38.5 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
2661 .. 3521  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2661 .. 3452  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2661 .. 3521  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3453 .. 3521  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2661 .. 3521  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1902 .. 2490  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1902 .. 2490  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3653 .. 4108  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3653 .. 4108  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
3522 .. 3626  =  105 bp
AmpR promoter
3522 .. 3626  =  105 bp
MCS
191 .. 286  =  96 bp
multiple cloning site
MCS
191 .. 286  =  96 bp
multiple cloning site
lacI promoter
1547 .. 1624  =  78 bp
lacI promoter
1547 .. 1624  =  78 bp
lac promoter
385 .. 415  =  31 bp
3 segments
   Segment 3:  -10  
   385 .. 391  =  7 bp
promoter for the E. coli lac operon
lac promoter
385 .. 415  =  31 bp
3 segments
   Segment 2:  
   392 .. 409  =  18 bp
promoter for the E. coli lac operon
lac promoter
385 .. 415  =  31 bp
3 segments
   Segment 1:  -35  
   410 .. 415  =  6 bp
promoter for the E. coli lac operon
lac promoter
385 .. 415  =  31 bp
3 segments
promoter for the E. coli lac operon
SP6 promoter
164 .. 182  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
164 .. 182  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
T7 promoter
300 .. 318  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
300 .. 318  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
140 .. 156  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
140 .. 156  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
337 .. 353  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
337 .. 353  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
361 .. 377  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
361 .. 377  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacZα
4085 .. 341  =  366 bp
121 amino acids  =  13.7 kDa
Product: LacZα fragment of β-galactosidase
lacZα
4085 .. 341  =  366 bp
121 amino acids  =  13.7 kDa
Product: LacZα fragment of β-galactosidase
ORF:  430 .. 681  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  2791 .. 3057  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4085 .. 341  =  366 bp
ORF:  121 amino acids  =  13.7 kDa
ORF:  444 .. 707  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  1296 .. 1613  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  2661 .. 3521  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  464 .. 1423  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
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