Primers & Probes for SARS-CoV-2 (COVID-19) Genome

Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. RT-PCR primers and probes for SARS-CoV-2 (COVID-19), assembled by Martin Reijns at the MRC Human Genetics Unit at the University of Edinburgh. UPDATED JAN 2021.

Sequence Author: Martin Reijns

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HomePlasmidsCoronavirus ResourcesPrimers & Probes for SARS-CoV-2 (COVID-19) Genome
25,000 20,000 15,000 10,000 5000 ORF10 2019-nCoV_N2-R (29,213 .. 29,230) 2019-nCoV_N2-P (29,188 .. 29,210) 2019-nCoV_N2-F (29,164 .. 29,183) N-R (28,958 .. 28,979) N-probe (28,934 .. 28,953) N-F (28,881 .. 28,902) 2019-nCoV_N1-R (28,335 .. 28,358) 2019-nCoV_N1-P (28,309 .. 28,332) 2019-nCoV_N1-F (28,287 .. 28,306) ORF8 ORF6 M-574-R (27,077 .. 27,096) M-507-P (27,029 .. 27,048) M-475-F (26,997 .. 27,016) E_Sarbeco_R2 (26,360 .. 26,381) E E_Sarbeco_P1 (26,332 .. 26,357) E_Sarbeco_F1 (26,269 .. 26,294) S-gene-R (21,796 .. 21,817) S-gene-P (21,759 .. 21,782) S‐Gene‐F (21,710 .. 21,731) RdRp_SARSr-R1 (15,505 .. 15,530) RdRp_SARSr-P2 (15,470 .. 15,494) RdRp_SARSr-F2 (15,431 .. 15,452) nCoV_IP4-14146Rv (14,167 .. 14,186) nCoV_IP4-14084Probe(+) (14,105 .. 14,123) nCoV_IP4-14059Fw (14,080 .. 14,098) ORF1ab-R (13,442 .. 13,460) ORF1ab-probe (13,377 .. 13,404) ORF1ab-F (13,342 .. 13,362) nCoV_IP2-12759Rv (12,780 .. 12,797) nCoV_IP2-12696bProbe(+) (12,717 .. 12,737) nCoV_IP2-12669Fw (12,690 .. 12,707) 5' UTR ORF1ab S ORF3a M ORF7a N 3' UTR ORF1ab mature peptide mature peptide ORF7b stem loop mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide stem loop mature peptide mature peptide mature peptide mature peptide mature peptide mature peptide 501_V2 lineage mutation C1059T (T265I) B.1.1.7 lineage 11288-11296 del (SGF 3675-3677del) stem loop