SARS Genome
Complete genome of the severe acute respiratory syndrome coronavirus (SARS).
Explore Over 2.7k Plasmids: Coronavirus Resources | More Plasmid Sets
No matches
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
| ||
Sticky ends from different EciI sites may not be compatible. |
|
|
|
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
|
| ||
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
| ||
Sticky ends from different TaqII sites may not be compatible. |
|
| 5' UTR 1 .. 264 = 264 bp |
| 5' UTR 1 .. 264 = 264 bp |
| orf1ab 265 .. 21,485 = 21,221 bp 7073 amino acids = 790.3 kDa 2 segments Segment 1: 265 .. 13,398 = 13,134 bp 4378 amino acids = 486.0 kDa Product: orf1ab polyprotein (pp1ab) It was assumed that the SARS orf1ab polyprotein processing map should be similar to that of murine hepatitis virus; however, of the two MHV papain-like proteinases, only PL2-PRO is well conserved for SARS coronavirus. The mature peptides located downstream from nsp4-pp1a/pp1ab are cleaved from the polyprotein by the nsp5-pp1a/pp1ab proteinase 3CL-PRO. The orf1a/orf1b translational frameshift, the predicted processing map, and both proteinase activities have been supported by in vitro expression and mutagenesis experiments (Thiel et al., 2003); -1 frameshift |
| orf1ab 265 .. 21,485 = 21,221 bp 7073 amino acids = 790.3 kDa 2 segments Segment 2: 13,398 .. 21,485 = 8088 bp 2695 amino acids = 304.3 kDa Product: orf1ab polyprotein (pp1ab) It was assumed that the SARS orf1ab polyprotein processing map should be similar to that of murine hepatitis virus; however, of the two MHV papain-like proteinases, only PL2-PRO is well conserved for SARS coronavirus. The mature peptides located downstream from nsp4-pp1a/pp1ab are cleaved from the polyprotein by the nsp5-pp1a/pp1ab proteinase 3CL-PRO. The orf1a/orf1b translational frameshift, the predicted processing map, and both proteinase activities have been supported by in vitro expression and mutagenesis experiments (Thiel et al., 2003); -1 frameshift |
| orf1ab 265 .. 21,485 = 21,221 bp 7073 amino acids = 790.3 kDa 2 segments Product: orf1ab polyprotein (pp1ab) It was assumed that the SARS orf1ab polyprotein processing map should be similar to that of murine hepatitis virus; however, of the two MHV papain-like proteinases, only PL2-PRO is well conserved for SARS coronavirus. The mature peptides located downstream from nsp4-pp1a/pp1ab are cleaved from the polyprotein by the nsp5-pp1a/pp1ab proteinase 3CL-PRO. The orf1a/orf1b translational frameshift, the predicted processing map, and both proteinase activities have been supported by in vitro expression and mutagenesis experiments (Thiel et al., 2003); -1 frameshift |
| transcription regulatory sequence for mRNA2 21,486 .. 21,491 = 6 bp transcription regulatory sequence for mRNA2 |
| transcription regulatory sequence for mRNA2 21,486 .. 21,491 = 6 bp transcription regulatory sequence for mRNA2 |
| S 21,492 .. 25,259 = 3768 bp 1255 amino acids = 139.1 kDa Product: E2 glycoprotein precursor As established by Krokhin et al. (2003), the glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients; spike glycoprotein |
| S 21,492 .. 25,259 = 3768 bp 1255 amino acids = 139.1 kDa Product: E2 glycoprotein precursor As established by Krokhin et al. (2003), the glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients; spike glycoprotein |
| transcription regulatory sequence for mRNA3 25,260 .. 25,265 = 6 bp transcription regulatory sequence for mRNA3 |
| transcription regulatory sequence for mRNA3 25,260 .. 25,265 = 6 bp transcription regulatory sequence for mRNA3 |
| sars3a 25,268 .. 26,092 = 825 bp 274 amino acids = 31.0 kDa Product: hypothetical protein sars3a |
| sars3a 25,268 .. 26,092 = 825 bp 274 amino acids = 31.0 kDa Product: hypothetical protein sars3a |
| transcription regulatory sequence for mRNA4 26,109 .. 26,114 = 6 bp transcription regulatory sequence for mRNA4 |
| transcription regulatory sequence for mRNA4 26,109 .. 26,114 = 6 bp transcription regulatory sequence for mRNA4 |
| E 26,117 .. 26,347 = 231 bp 76 amino acids = 8.4 kDa Product: protein E E. coli expression reported by Shen et al. (2003); protein sM; small envelope protein |
| E 26,117 .. 26,347 = 231 bp 76 amino acids = 8.4 kDa Product: protein E E. coli expression reported by Shen et al. (2003); protein sM; small envelope protein |
| transcription regulatory sequence for mRNA5 26,348 .. 26,353 = 6 bp transcription regulatory sequence for mRNA5 |
| transcription regulatory sequence for mRNA5 26,348 .. 26,353 = 6 bp transcription regulatory sequence for mRNA5 |
| M 26,398 .. 27,063 = 666 bp 221 amino acids = 25.1 kDa Product: matrix protein E. coli expression reported by Zhang et al. (2003) |
| M 26,398 .. 27,063 = 666 bp 221 amino acids = 25.1 kDa Product: matrix protein E. coli expression reported by Zhang et al. (2003) |
| sars6 27,074 .. 27,265 = 192 bp 63 amino acids = 7.5 kDa Product: hypothetical protein sars6 |
| sars6 27,074 .. 27,265 = 192 bp 63 amino acids = 7.5 kDa Product: hypothetical protein sars6 |
| transcription regulatory sequence for mRNA7 27,267 .. 27,272 = 6 bp transcription regulatory sequence for mRNA7 |
| transcription regulatory sequence for mRNA7 27,267 .. 27,272 = 6 bp transcription regulatory sequence for mRNA7 |
| sars7a 27,273 .. 27,641 = 369 bp 122 amino acids = 13.9 kDa Product: hypothetical protein sars7a |
| sars7a 27,273 .. 27,641 = 369 bp 122 amino acids = 13.9 kDa Product: hypothetical protein sars7a |
| transcription regulatory sequence for mRNA8 27,773 .. 27,778 = 6 bp transcription regulatory sequence for mRNA8 |
| transcription regulatory sequence for mRNA8 27,773 .. 27,778 = 6 bp transcription regulatory sequence for mRNA8 |
| sars8b 27,864 .. 28,118 = 255 bp 84 amino acids = 9.6 kDa Product: hypothetical protein sars8b |
| sars8b 27,864 .. 28,118 = 255 bp 84 amino acids = 9.6 kDa Product: hypothetical protein sars8b |
| N 28,120 .. 29,388 = 1269 bp 422 amino acids = 46.0 kDa Product: nucleocapsid protein As established by Krokhin et al. (2003), the N-terminal methionine is removed, all other methionines are oxidized, and the resulting N-terminal serine is acetylated |
| N 28,120 .. 29,388 = 1269 bp 422 amino acids = 46.0 kDa Product: nucleocapsid protein As established by Krokhin et al. (2003), the N-terminal methionine is removed, all other methionines are oxidized, and the resulting N-terminal serine is acetylated |
| 3' UTR 29,389 .. 29,751 = 363 bp |
| 3' UTR 29,389 .. 29,751 = 363 bp |
| transcription regulatory sequence leader TRS 67 .. 72 = 6 bp transcription regulatory sequence leader TRS |
| transcription regulatory sequence leader TRS 67 .. 72 = 6 bp transcription regulatory sequence leader TRS |
| orf1ab 265 .. 13,413 = 13,149 bp 4382 amino acids = 486.4 kDa Product: orf1a polyprotein (pp1a) |
| orf1ab 265 .. 13,413 = 13,149 bp 4382 amino acids = 486.4 kDa Product: orf1a polyprotein (pp1a) |
| mature peptide 16,167 .. 17,969 = 1803 bp Product: nsp13-pp1ab (ZD, NTPase/HEL; RNA 5'-triphosphatase) zinc-binding domain (ZD), NTPase/helicase domain. RNA-stimulated ATPase and dsDNA helicase activities have been confirmed (Thiel et al., 2003) /protein_id=NP_828870.1 |
| mature peptide 16,167 .. 17,969 = 1803 bp Product: nsp13-pp1ab (ZD, NTPase/HEL; RNA 5'-triphosphatase) zinc-binding domain (ZD), NTPase/helicase domain. RNA-stimulated ATPase and dsDNA helicase activities have been confirmed (Thiel et al., 2003) /protein_id=NP_828870.1 |
| mature peptide 17,970 .. 19,550 = 1581 bp Product: 3'-to-5' exonuclease nsp14-pp1ab (nuclease ExoN homolog) /protein_id=NP_828871.1 |
| mature peptide 17,970 .. 19,550 = 1581 bp Product: 3'-to-5' exonuclease nsp14-pp1ab (nuclease ExoN homolog) /protein_id=NP_828871.1 |
| mature peptide 19,551 .. 20,588 = 1038 bp Product: endoRNAse the C-terminal domain is a homolog of endoRNase XendoU and is conserved through the order Nidovirales; nsp15-pp1ab; uridylate-specific endoribonuclease NendoU /protein_id=NP_828872.1 |
| mature peptide 19,551 .. 20,588 = 1038 bp Product: endoRNAse the C-terminal domain is a homolog of endoRNase XendoU and is conserved through the order Nidovirales; nsp15-pp1ab; uridylate-specific endoribonuclease NendoU /protein_id=NP_828872.1 |
| mature peptide 20,589 .. 21,482 = 894 bp Product: 2'-O-ribose methyltransferase (2'-o-MT) nsp16-pp1ab /protein_id=NP_828873.2 |
| mature peptide 20,589 .. 21,482 = 894 bp Product: 2'-O-ribose methyltransferase (2'-o-MT) nsp16-pp1ab /protein_id=NP_828873.2 |
| second glycosylation site 21,843 .. 21,845 = 3 bp second glycosylation site |
| second glycosylation site 21,843 .. 21,845 = 3 bp second glycosylation site |
| first glycosylation site 21,846 .. 21,848 = 3 bp first glycosylation site |
| first glycosylation site 21,846 .. 21,848 = 3 bp first glycosylation site |
| glycosylation site 22,170 .. 22,172 = 3 bp glycosylation site |
| glycosylation site 22,170 .. 22,172 = 3 bp glycosylation site |
| glycosylation site 22,296 .. 22,298 = 3 bp glycosylation site |
| glycosylation site 22,296 .. 22,298 = 3 bp glycosylation site |
| glycosylation site 23,838 .. 23,840 = 3 bp glycosylation site |
| glycosylation site 23,838 .. 23,840 = 3 bp glycosylation site |
| sars3b 25,689 .. 26,153 = 465 bp 154 amino acids = 17.8 kDa Product: hypothetical protein sars3b |
| sars3b 25,689 .. 26,153 = 465 bp 154 amino acids = 17.8 kDa Product: hypothetical protein sars3b |
| sars6 26,913 .. 27,265 = 353 bp |
| sars6 26,913 .. 27,265 = 353 bp |
| sars7b 27,638 .. 27,772 = 135 bp 44 amino acids = 5.3 kDa Product: hypothetical protein sars7b |
| sars7b 27,638 .. 27,772 = 135 bp 44 amino acids = 5.3 kDa Product: hypothetical protein sars7b |
| sars8a 27,779 .. 27,898 = 120 bp 39 amino acids = 4.3 kDa Product: hypothetical protein sars8a |
| sars8a 27,779 .. 27,898 = 120 bp 39 amino acids = 4.3 kDa Product: hypothetical protein sars8a |
| transcription regulatory sequence for mRNA9 28,106 .. 28,111 = 6 bp transcription regulatory sequence for mRNA9 |
| transcription regulatory sequence for mRNA9 28,106 .. 28,111 = 6 bp transcription regulatory sequence for mRNA9 |
| acetylation site 28,123 .. 28,125 = 3 bp acetylation site |
| acetylation site 28,123 .. 28,125 = 3 bp acetylation site |
| sars9b 28,130 .. 28,426 = 297 bp 98 amino acids = 10.8 kDa Product: hypothetical protein sars9b |
| sars9b 28,130 .. 28,426 = 297 bp 98 amino acids = 10.8 kDa Product: hypothetical protein sars9b |
| mature peptide 265 .. 804 = 540 bp Product: leader protein PL2-PRO cleavage product; nsp1-pp1a/pp1ab /protein_id=NP_828860.2 |
| mature peptide 265 .. 804 = 540 bp Product: leader protein PL2-PRO cleavage product; nsp1-pp1a/pp1ab /protein_id=NP_828860.2 |
| mature peptide 805 .. 2718 = 1914 bp Product: counterpart of MHV p65 PL2-PRO cleavage product; nsp2-pp1a/pp1ab /protein_id=NP_828861.2 |
| mature peptide 805 .. 2718 = 1914 bp Product: counterpart of MHV p65 PL2-PRO cleavage product; nsp2-pp1a/pp1ab /protein_id=NP_828861.2 |
| mature peptide 2719 .. 8484 = 5766 bp Product: nsp3-pp1a/pp1ab PL2-PRO cleavage product; former nsp1; conserved domains are: N-terminal acidic (Ac), predicted phosphoesterase (similar to the Appr-1'-p; processing enzyme) formerly known as 'X-domain', papain-like proteinase similar to that of MHV PL2-PRO, Y-domain; transmembrane domain 1 (TM1); adenosine diphosphate-ribose 1''-phosphatase (ADPR) /protein_id=NP_828862.2 |
| mature peptide 2719 .. 8484 = 5766 bp Product: nsp3-pp1a/pp1ab PL2-PRO cleavage product; former nsp1; conserved domains are: N-terminal acidic (Ac), predicted phosphoesterase (similar to the Appr-1'-p; processing enzyme) formerly known as 'X-domain', papain-like proteinase similar to that of MHV PL2-PRO, Y-domain; transmembrane domain 1 (TM1); adenosine diphosphate-ribose 1''-phosphatase (ADPR) /protein_id=NP_828862.2 |
| mature peptide 8485 .. 9984 = 1500 bp Product: nsp4-pp1a/pp1ab cleaved from polyprotein by the PL2-PRO at the N-terminus and by 3CL-PRO at the C-terminus; contains transmembrane domain 2 (TM2) /protein_id=NP_904322.1 |
| mature peptide 8485 .. 9984 = 1500 bp Product: nsp4-pp1a/pp1ab cleaved from polyprotein by the PL2-PRO at the N-terminus and by 3CL-PRO at the C-terminus; contains transmembrane domain 2 (TM2) /protein_id=NP_904322.1 |
| mature peptide 9985 .. 10,902 = 918 bp Product: 3C-like proteinase mediates cleavages downstream from nsp4-pp1a/pp1ab. 3D structure has been determined (Yang et al., 2003); main proteinase (Mpro); nsp5-pp1a/pp1ab (3CL-PRO) /protein_id=NP_828863.1 |
| mature peptide 9985 .. 10,902 = 918 bp Product: 3C-like proteinase mediates cleavages downstream from nsp4-pp1a/pp1ab. 3D structure has been determined (Yang et al., 2003); main proteinase (Mpro); nsp5-pp1a/pp1ab (3CL-PRO) /protein_id=NP_828863.1 |
| mature peptide 10,903 .. 11,772 = 870 bp Product: nsp6-pp1a/pp1ab (TM3) putative transmembrane domain /protein_id=NP_828864.1 |
| mature peptide 10,903 .. 11,772 = 870 bp Product: nsp6-pp1a/pp1ab (TM3) putative transmembrane domain /protein_id=NP_828864.1 |
| mature peptide 11,773 .. 12,021 = 249 bp Product: nsp7-pp1a/pp1ab /protein_id=NP_828865.1 |
| mature peptide 11,773 .. 12,021 = 249 bp Product: nsp7-pp1a/pp1ab /protein_id=NP_828865.1 |
| mature peptide 12,022 .. 12,615 = 594 bp Product: nsp8-pp1a/pp1ab /protein_id=NP_828866.1 |
| mature peptide 12,022 .. 12,615 = 594 bp Product: nsp8-pp1a/pp1ab /protein_id=NP_828866.1 |
| mature peptide 12,616 .. 12,954 = 339 bp Product: nsp9-pp1a/pp1ab ssRNA-binding protein /protein_id=NP_828867.1 |
| mature peptide 12,616 .. 12,954 = 339 bp Product: nsp9-pp1a/pp1ab ssRNA-binding protein /protein_id=NP_828867.1 |
| mature peptide 12,955 .. 13,371 = 417 bp Product: formerly known as growth-factor-like protein (GFL) nsp10-pp1a/pp1ab /protein_id=NP_828868.1 |
| mature peptide 12,955 .. 13,371 = 417 bp Product: formerly known as growth-factor-like protein (GFL) nsp10-pp1a/pp1ab /protein_id=NP_828868.1 |
| mature peptide 13,372 .. 16,166 = 2795 bp 2 segments Segment 1: 13,372 .. 13,398 = 27 bp Product: RNA-dependent RNA polymerase nsp12-pp1ab (RdRp) /protein_id=NP_828869.1 |
| mature peptide 13,372 .. 16,166 = 2795 bp 2 segments Segment 2: 13,398 .. 16,166 = 2769 bp Product: RNA-dependent RNA polymerase nsp12-pp1ab (RdRp) /protein_id=NP_828869.1 |
| mature peptide 13,372 .. 16,166 = 2795 bp 2 segments Product: RNA-dependent RNA polymerase nsp12-pp1ab (RdRp) /protein_id=NP_828869.1 |
| transcription regulatory sequence for mRNA6 26,913 .. 26,918 = 6 bp transcription regulatory sequence for mRNA6 |
| transcription regulatory sequence for mRNA6 26,913 .. 26,918 = 6 bp transcription regulatory sequence for mRNA6 |
| miscellaneous 13,392 .. 13,472 = 81 bp Region: potential ribosome slippery sequence followed by stimulatory RNA pseudoknot |
| miscellaneous 13,392 .. 13,472 = 81 bp Region: potential ribosome slippery sequence followed by stimulatory RNA pseudoknot |
| mature peptide 13,372 .. 13,410 = 39 bp Product: nsp11-pp1a putative C-terminal cleavage product of pp1a /protein_id=NP_904321.1 |
| mature peptide 13,372 .. 13,410 = 39 bp Product: nsp11-pp1a putative C-terminal cleavage product of pp1a /protein_id=NP_904321.1 |
| ORF: 265 .. 13,413 = 13,149 bp ORF: 4382 amino acids = 486.4 kDa |
| ORF: 26,398 .. 27,063 = 666 bp ORF: 221 amino acids = 25.1 kDa |
| ORF: 28,120 .. 29,388 = 1269 bp ORF: 422 amino acids = 46.0 kDa |
| ORF: 734 .. 1225 = 492 bp ORF: 163 amino acids = 17.7 kDa |
| ORF: 2993 .. 3295 = 303 bp ORF: 100 amino acids = 12.0 kDa |
| ORF: 10,067 .. 10,330 = 264 bp ORF: 87 amino acids = 10.0 kDa |
| ORF: 25,268 .. 26,092 = 825 bp ORF: 274 amino acids = 31.0 kDa |
| ORF: 26,117 .. 26,347 = 231 bp ORF: 76 amino acids = 8.4 kDa |
| ORF: 28,130 .. 28,426 = 297 bp ORF: 98 amino acids = 10.8 kDa |
| ORF: 13,599 .. 21,485 = 7887 bp ORF: 2628 amino acids = 296.8 kDa |
| ORF: 21,492 .. 25,259 = 3768 bp ORF: 1255 amino acids = 139.1 kDa |
| ORF: 25,689 .. 26,153 = 465 bp ORF: 154 amino acids = 17.8 kDa |
| ORF: 27,273 .. 27,641 = 369 bp ORF: 122 amino acids = 13.9 kDa |
| ORF: 27,864 .. 28,118 = 255 bp ORF: 84 amino acids = 9.6 kDa |
| ORF: 1788 .. 2015 = 228 bp ORF: 75 amino acids = 8.0 kDa |
| ORF: 8631 .. 8879 = 249 bp ORF: 82 amino acids = 8.9 kDa |
| ORF: 18,807 .. 19,088 = 282 bp ORF: 93 amino acids = 11.2 kDa |
| ORF: 19,554 .. 19,835 = 282 bp ORF: 93 amino acids = 10.8 kDa |
| ORF: 21,752 .. 21,982 = 231 bp ORF: 76 amino acids = 8.4 kDa |
| Click here to try SnapGene |
Download SARS Genome.dna file
SnapGene
SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures
- Fast accurate construct design for all major molecular cloning techniques
- Validate sequenced constructs using powerful alignment tools
- Customize plasmid maps with flexible annotation and visualization controls
- Automatically generate a rich graphical history of every edit and procedure
SnapGene Viewer
SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.
- Gain unparalleled visibility of your plasmids, DNA and protein sequences
- Annotate features on your plasmids using the curated feature database
- Store, search, and share your sequences, files and maps
Individual Sequences & Maps
Plasmid Sets
The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.
Discover the most user-friendly molecular biology experience.